Bergamottin, a natural furanocoumarin abundantly present in grapefruit juice, suppresses the invasiveness of human glioma cells via inactivation of Rac1 signaling

  • Authors:
    • Wenzheng Luo
    • Zhenyu Song
    • Hongwei Sun
    • Junxin Liang
    • Shanshan Zhao
  • View Affiliations

  • Published online on: December 19, 2017     https://doi.org/10.3892/ol.2017.7641
  • Pages: 3259-3266
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Abstract

The aim of the present study was to explore the effect of bergamottin, a natural furanocoumarin obtained from grapefruit juice, on the invasiveness of human glioma cells. The results revealed that treatment with bergamottin for 48 h significantly inhibited wound‑healing migration and Matrigel invasion of human glioma cells, compared with untreated cells (P<0.05). Bergamottin treatment caused a significant decrease in the expression and secretion of matrix metalloproteinase (MMP)‑9 in glioma cells compared with untreated cells (P<0.05). A Rac1‑GTP pull‑down assay demonstrated that bergamottin‑treated glioma cells had a significantly decreased level of active Rac1‑GTP compared with untreated cells (P<0.05). However, bergamottin had no significant effect on cell division cycle 42 activity. Expression of constitutively activated Rac1 almost completely restored the migration and invasion of bergamottin‑treated glioma cells. In addition, bergamottin‑induced downregulation of MMP‑9 was prevented by exogenous activated Rac1. The results of the present study demonstrated that bergamottin exhibits anti‑invasive activity in human glioma cells through the inactivation of Rac1 and downregulation of MMP‑9.
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March-2018
Volume 15 Issue 3

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Luo W, Song Z, Sun H, Liang J and Zhao S: Bergamottin, a natural furanocoumarin abundantly present in grapefruit juice, suppresses the invasiveness of human glioma cells via inactivation of Rac1 signaling. Oncol Lett 15: 3259-3266, 2018
APA
Luo, W., Song, Z., Sun, H., Liang, J., & Zhao, S. (2018). Bergamottin, a natural furanocoumarin abundantly present in grapefruit juice, suppresses the invasiveness of human glioma cells via inactivation of Rac1 signaling. Oncology Letters, 15, 3259-3266. https://doi.org/10.3892/ol.2017.7641
MLA
Luo, W., Song, Z., Sun, H., Liang, J., Zhao, S."Bergamottin, a natural furanocoumarin abundantly present in grapefruit juice, suppresses the invasiveness of human glioma cells via inactivation of Rac1 signaling". Oncology Letters 15.3 (2018): 3259-3266.
Chicago
Luo, W., Song, Z., Sun, H., Liang, J., Zhao, S."Bergamottin, a natural furanocoumarin abundantly present in grapefruit juice, suppresses the invasiveness of human glioma cells via inactivation of Rac1 signaling". Oncology Letters 15, no. 3 (2018): 3259-3266. https://doi.org/10.3892/ol.2017.7641