MicroRNA‑21‑5p induces the metastatic phenotype of human cervical carcinoma cells in vitro by targeting the von Hippel‑Lindau tumor suppressor

  • Authors:
    • Lina Cai
    • Wuliang Wang
    • Xiaomei Li
    • Tieli Dong
    • Qing Zhang
    • Baojv Zhu
    • Hu Zhao
    • Shubiao Wu
  • View Affiliations

  • Published online on: February 2, 2018     https://doi.org/10.3892/ol.2018.7937
  • Pages: 5213-5219
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Abstract

Numerous studies have indicated that microRNAs (miRs), a group of small non‑coding RNAs, are determining regulatory elements involved in the pathogenesis of various types of cancer, including cervical cancer (CC). Although miR‑21‑5p upregulation has been demonstrated to associate with tumorigenesis by controlling the expression of oncogenic and tumor suppressor genes, only a small number of studies have investigated the expression of miR‑21‑5p and its functional role in CC. The objective of the present study was to investigate the effect of miR‑21‑5p on the proliferation, apoptosis, migration and invasion of CC cells, and the potential underlying molecular mechanism of these effects. The measurement of miR‑21‑5p levels using quantitative polymerase chain reaction revealed that miR‑21‑5p was markedly increased in CC cell lines compared with normal cells. Upon silencing of miR‑21‑5p, a marked suppression of the proliferation, migration and invasion of CaSki cells was observed, with induction of cell apoptosis. These effects were reversed with miR‑21‑5p overexpression. A database search followed by a luciferase reporter assay ascertained that the 3'‑untranslated region of the von Hippel‑Lindau tumor suppressor (VHL) mRNA sequence was a direct target of miR‑21‑5p. Furthermore, silencing of VHL neutralized the effects of miR‑21‑5p inhibition. These observations suggested that miR‑21‑5p is an oncogene that is able to promote the metastatic phenotype of CC cells through downregulation of VHL expression, which may present a path to novel therapeutic stratagems for the CC therapy.
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April-2018
Volume 15 Issue 4

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Cai L, Wang W, Li X, Dong T, Zhang Q, Zhu B, Zhao H and Wu S: MicroRNA‑21‑5p induces the metastatic phenotype of human cervical carcinoma cells in vitro by targeting the von Hippel‑Lindau tumor suppressor. Oncol Lett 15: 5213-5219, 2018
APA
Cai, L., Wang, W., Li, X., Dong, T., Zhang, Q., Zhu, B. ... Wu, S. (2018). MicroRNA‑21‑5p induces the metastatic phenotype of human cervical carcinoma cells in vitro by targeting the von Hippel‑Lindau tumor suppressor. Oncology Letters, 15, 5213-5219. https://doi.org/10.3892/ol.2018.7937
MLA
Cai, L., Wang, W., Li, X., Dong, T., Zhang, Q., Zhu, B., Zhao, H., Wu, S."MicroRNA‑21‑5p induces the metastatic phenotype of human cervical carcinoma cells in vitro by targeting the von Hippel‑Lindau tumor suppressor". Oncology Letters 15.4 (2018): 5213-5219.
Chicago
Cai, L., Wang, W., Li, X., Dong, T., Zhang, Q., Zhu, B., Zhao, H., Wu, S."MicroRNA‑21‑5p induces the metastatic phenotype of human cervical carcinoma cells in vitro by targeting the von Hippel‑Lindau tumor suppressor". Oncology Letters 15, no. 4 (2018): 5213-5219. https://doi.org/10.3892/ol.2018.7937