Correlation of hypoxia status with radiosensitizing effects of sodium glycididazole: A preclinical study

  • Authors:
    • Yang Yu
    • Xiaolin Li
    • Hengwei Xu
    • Jing Liu
    • Min Dong
    • Jia Yang
    • Lu Sun
    • Xiaorong Sun
    • Ligang Xing
  • View Affiliations

  • Published online on: February 21, 2018     https://doi.org/10.3892/ol.2018.8096
  • Pages: 6481-6488
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

The correlation of pretreatment hypoxia status with the radiosensitization effect of sodium glycididazole (CMNa) was not previously defined. The purpose of the present study was to evaluate the tumor hypoxia status in various cancer xenografts and to investigate the correlation between tumor hypoxia status and radiosensitizing effects of CMNa based on the pharmacokinetic and pharmacodynamic parameters. Human esophageal cancer (EC109), head and neck cancer (FaDu) and lung cancer (A549) nude mice xenografts were used. The concentrations of CMNa and its metabolites in the tumors and normal tissues were determined by high‑performance liquid chromatography following intravenous injection of 171.9, 57.3 or 19.1 mg/kg CMNa. The tumors were irradiated with 30 Gy in 6 fractions with CMNa administration prior to each irradiation. The tumor growth delay values were calculated for each treatment group and compared with groups treated with radiation alone. Tumor hypoxia status was verified by immunohistostaining of tissues for hypoxia inducible factor 1α (HIF‑1α) staining, and the concentration of plasma osteopontin (OPN) was determined using ELISA. The correlation between OPN concentration and tumor growth delay was subsequently analyzed. It was observed that the drug concentration in the tumor was 1.6‑2.8 times higher compared with adjacent muscle, particularly at high and medium doses. CMNa was able to sensitize tumors to irradiation, particularly for EC109 and FaDu xenografts at high dose (P<0.05). Furthermore, there was markedly increased expression of HIF‑1α and plasma OPN levels in FaDu and EC109 xenografts compared with A549. Additionally, it was indicated that pretreatment hypoxia status might be correlated with the radiosensitizing effects of CMNa. The present data demonstrated that tumor hypoxia status might be correlated with the radiosensitizing effects of CMNa in different tumor models.
View Figures
View References

Related Articles

Journal Cover

May-2018
Volume 15 Issue 5

Print ISSN: 1792-1074
Online ISSN:1792-1082

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Yu Y, Li X, Xu H, Liu J, Dong M, Yang J, Sun L, Sun X and Xing L: Correlation of hypoxia status with radiosensitizing effects of sodium glycididazole: A preclinical study. Oncol Lett 15: 6481-6488, 2018
APA
Yu, Y., Li, X., Xu, H., Liu, J., Dong, M., Yang, J. ... Xing, L. (2018). Correlation of hypoxia status with radiosensitizing effects of sodium glycididazole: A preclinical study. Oncology Letters, 15, 6481-6488. https://doi.org/10.3892/ol.2018.8096
MLA
Yu, Y., Li, X., Xu, H., Liu, J., Dong, M., Yang, J., Sun, L., Sun, X., Xing, L."Correlation of hypoxia status with radiosensitizing effects of sodium glycididazole: A preclinical study". Oncology Letters 15.5 (2018): 6481-6488.
Chicago
Yu, Y., Li, X., Xu, H., Liu, J., Dong, M., Yang, J., Sun, L., Sun, X., Xing, L."Correlation of hypoxia status with radiosensitizing effects of sodium glycididazole: A preclinical study". Oncology Letters 15, no. 5 (2018): 6481-6488. https://doi.org/10.3892/ol.2018.8096