EGFR-TKI resistance and MAP17 are associated with cancer stem cell like properties

  • Authors:
    • Yi Shao
    • Hui Lv
    • Dian‑Sheng Zhong
    • Qing‑Hua Zhou
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  • Published online on: February 27, 2018     https://doi.org/10.3892/ol.2018.8129
  • Pages: 6655-6665
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Abstract

Patients with non-small-cell lung cancer (NSCLC) with sensitive epidermal growth factor receptor (EGFR) mutations generally react well to tyrosine kinase inhibitors (TKIs). However acquired resistance eventually occurs. Several mechanisms contribute to the resistance including T790M mutation, c‑Met amplification and PIK3CA mutation. In recent years, cancer stem cells (CSCs) have been suggested to be involved in TKI resistance. MAP17 is aberrantly overexpressed in a number of malignancies. However, the expression pattern and function of MAP17 in CSCs are still unclear. The aim the present study was to illustrate the effect of CSC‑like cells on the resistance to TKIs in EGFR mutant NSCLC cells and explore the possible role of MAP17 in CSCs. The EGFR mutant cell line PC9 was cultured under serum‑deprived undifferentiated conditions. The CSC properties including expression of stem cell markers CD133, CD44, Oct‑4 and ABCG2, ability of self‑renewal, invasion, proliferation and tumorigenesis were examined. The expression of MAP17 was compared in sphere and parent cells. Sphere cells displayed stem cells phenotypes and were resistant to erlotinib. Sphere cells expressed higher levels of MAP17, and MAP17 was associated with self‑renewal and TKI resistance. The function of MAP17 demonstrated to be partially dependent on Na‑dependent glucose transporter 1. Collectively these findings suggest that MAP17 serves a role in TKI resistance through regulation of CSCs in lung cancer.
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May-2018
Volume 15 Issue 5

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Shao Y, Lv H, Zhong DS and Zhou QH: EGFR-TKI resistance and MAP17 are associated with cancer stem cell like properties. Oncol Lett 15: 6655-6665, 2018
APA
Shao, Y., Lv, H., Zhong, D., & Zhou, Q. (2018). EGFR-TKI resistance and MAP17 are associated with cancer stem cell like properties. Oncology Letters, 15, 6655-6665. https://doi.org/10.3892/ol.2018.8129
MLA
Shao, Y., Lv, H., Zhong, D., Zhou, Q."EGFR-TKI resistance and MAP17 are associated with cancer stem cell like properties". Oncology Letters 15.5 (2018): 6655-6665.
Chicago
Shao, Y., Lv, H., Zhong, D., Zhou, Q."EGFR-TKI resistance and MAP17 are associated with cancer stem cell like properties". Oncology Letters 15, no. 5 (2018): 6655-6665. https://doi.org/10.3892/ol.2018.8129