LINE-1 hypomethylation is inversely correlated with UHRF1 overexpression in gastric cancer

  • Authors:
    • Jang Hee Hong
    • Eun‑Heui Jin
    • Soyeon Kim
    • Kyu‑Sang Song
    • Jae Kyu Sung
  • View Affiliations

  • Published online on: February 27, 2018     https://doi.org/10.3892/ol.2018.8121
  • Pages: 6666-6670
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Abstract

DNA methylation is an important epigenetic modification that alters gene expression; DNA hypomethylation contributes to tumorigenesis through multiple processes. In the present study, the methylation of long interspersed element-1 (LINE-1) in 95 gastric cancer (GC) tissues and matched adjacent normal tissues was investigated by pyrosequencing. LINE‑1 methylation was compared with the expression of ubiquitin‑like with PHD and ring‑finger protein 1 (UHRF1), an essential regulator of DNA methylation, using reverse transcription‑quantitative polymerase chain reaction. Significant hypomethylation of LINE‑1 and overexpression of UHRF1 were observed in GC tissues compared with the matched controls (P<0.001 and P=0.001, respectively). LINE‑1 hypomethylation was inversely correlated with UHRF1 overexpression in GC tissues (r=‑0.026, P=0.028). In addition, LINE‑1 hypomethylation in GC was significantly associated with Lauren's histological classification, tumor differentiation and background intestinal metaplasia (P=0.014, P=0.042 and P=0.034, respectively). These results suggest that LINE‑1 hypomethylation may be a potential biomarker for GC and it is indirectly regulated by UHRF1 overexpression.
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May-2018
Volume 15 Issue 5

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Hong JH, Jin EH, Kim S, Song KS and Sung JK: LINE-1 hypomethylation is inversely correlated with UHRF1 overexpression in gastric cancer. Oncol Lett 15: 6666-6670, 2018
APA
Hong, J.H., Jin, E., Kim, S., Song, K., & Sung, J.K. (2018). LINE-1 hypomethylation is inversely correlated with UHRF1 overexpression in gastric cancer. Oncology Letters, 15, 6666-6670. https://doi.org/10.3892/ol.2018.8121
MLA
Hong, J. H., Jin, E., Kim, S., Song, K., Sung, J. K."LINE-1 hypomethylation is inversely correlated with UHRF1 overexpression in gastric cancer". Oncology Letters 15.5 (2018): 6666-6670.
Chicago
Hong, J. H., Jin, E., Kim, S., Song, K., Sung, J. K."LINE-1 hypomethylation is inversely correlated with UHRF1 overexpression in gastric cancer". Oncology Letters 15, no. 5 (2018): 6666-6670. https://doi.org/10.3892/ol.2018.8121