Open Access

Overexpression of ATP citrate lyase in renal cell carcinoma tissues and its effect on the human renal carcinoma cells in vitro

  • Authors:
    • Lichen Teng
    • Yongsheng Chen
    • Yan Cao
    • Wentao Wang
    • Yongpeng Xu
    • Yanjie Wang
    • Jiayin Lv
    • Changfu Li
    • Yajuan Su
  • View Affiliations

  • Published online on: March 8, 2018     https://doi.org/10.3892/ol.2018.8211
  • Pages: 6967-6974
  • Copyright: © Teng et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

ATP citrate lyase (ACLY) is a key enzyme of lipogenesis in cells. However, ACLY expression in renal cell carcinoma (RCC) and its association with clinicopathological parameters remain unclear. The present study aimed to evaluate ACLY expression levels in RCC and adjacent normal tissues. This study included 33 patients with clear cell RCC (ccRCC). ACLY protein was assayed using immunohistochemistry and western blotting methods. ACLY mRNA expression was determined by reverse transcription‑quantitative polymerase chain reaction. Serum ACLY concentrations were measured using the ELISA. Compared with adjacent normal tissues, significantly higher levels of ACLY protein expression were observed in all of the ccRCC tissues (P<0.05). ACLY protein levels were positively associated with the T stage and nuclear grade of RCC. ACLY immunostaining was located in the cytoplasm and nucleus. ACLY protein levels and ACC1 mRNA expression in RCC tissues were significantly higher compared with that in adjacent normal tissues (P<0.05). There were no significant differences in serum ACLY concentrations between patients with RCC and health controls (P>0.05). Preliminary evaluation of ACLY function showed that ACLY small interfering RNA downregulation inhibited RCC cell proliferation and migration, but promoted RCC cell apoptosis. ACLY may be a novel biomarker for the evaluation of biological aggressiveness and may be a potential target for RCC treatment.
View Figures
View References

Related Articles

Journal Cover

May-2018
Volume 15 Issue 5

Print ISSN: 1792-1074
Online ISSN:1792-1082

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Teng L, Chen Y, Cao Y, Wang W, Xu Y, Wang Y, Lv J, Li C and Su Y: Overexpression of ATP citrate lyase in renal cell carcinoma tissues and its effect on the human renal carcinoma cells in vitro. Oncol Lett 15: 6967-6974, 2018
APA
Teng, L., Chen, Y., Cao, Y., Wang, W., Xu, Y., Wang, Y. ... Su, Y. (2018). Overexpression of ATP citrate lyase in renal cell carcinoma tissues and its effect on the human renal carcinoma cells in vitro. Oncology Letters, 15, 6967-6974. https://doi.org/10.3892/ol.2018.8211
MLA
Teng, L., Chen, Y., Cao, Y., Wang, W., Xu, Y., Wang, Y., Lv, J., Li, C., Su, Y."Overexpression of ATP citrate lyase in renal cell carcinoma tissues and its effect on the human renal carcinoma cells in vitro". Oncology Letters 15.5 (2018): 6967-6974.
Chicago
Teng, L., Chen, Y., Cao, Y., Wang, W., Xu, Y., Wang, Y., Lv, J., Li, C., Su, Y."Overexpression of ATP citrate lyase in renal cell carcinoma tissues and its effect on the human renal carcinoma cells in vitro". Oncology Letters 15, no. 5 (2018): 6967-6974. https://doi.org/10.3892/ol.2018.8211