Open Access

Overexpression of hsa‑miR‑125a‑5p enhances proliferation, migration and invasion of head and neck squamous cell carcinoma cell lines by upregulating C‑C chemokine receptor type 7

  • Authors:
    • Shan Jin
    • Min‑Da Liu
    • Hong Wu
    • Pai Pang
    • Song Wang
    • Zhen‑Ning Li
    • Chang‑Fu Sun
    • Fa‑Yu Liu
  • View Affiliations

  • Published online on: April 25, 2018     https://doi.org/10.3892/ol.2018.8564
  • Pages: 9703-9710
  • Copyright: © Jin et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Head and neck squamous cell carcinoma (HNSCC) is usually diagnosed accompanied by lymph node metastasis. C‑C chemokine receptor type 7 (CCR7) is associated with the invasion and metastasis of tumors in HNSCC through various signaling pathways. The role of hsa‑miR‑125a‑5p in HNSCC remains unclear. The present study was performed to investigate the association between hsa‑miR‑125a‑5p and CCR7 in HNSCC. Reverse transcription‑quantitative polymerase chain reaction was applied to analyze the expression of hsa‑miR‑125a‑5p in clinical samples. Cell Counting Kit‑8, Transwell and wound healing assays were used to detect cell proliferation, invasion, and metastasis, respectively, following overexpression of hsa‑miR‑125a‑5p. Changes in protein expression of CCR7 were observed using western blotting. In the survival analysis, Student's t‑tests and log rank tests were performed to analyze the association between the expression of hsa‑miR‑125a‑5p, and HNSCC according to the Cancer Genome Atlas database. The expression of hsa‑miR‑125a‑5p was identified to be significantly lower in cancer tissue compared with the corresponding adjacent normal tissues in clinical samples (P=0.038). The results of western blotting indicated that there was a positive regulatory association between hsa‑miR‑125a‑5p and CCR7. Furthermore, overexpression of hsa‑miR‑125a‑5p significantly enhanced the ability of cell proliferation, migration and invasion in HNSCC, with upregulation of CCR7. The results of survival analysis revealed that patients in the low expression group of hsa‑miR‑125a‑5p tended to have longer survival times compared with the high expression group (P=0.045). Altogether, the data raised the possibility that hsa‑miR‑125a‑5p has a significant role in promoting cancer in HNSCC, which may provide a basis for the treatment of HNSCC in molecular targeted therapy. Further studies are required to ascertain the role of hsa‑miR‑125a‑5p in other HNSCC cell lines and in vivo.
View Figures
View References

Related Articles

Journal Cover

June-2018
Volume 15 Issue 6

Print ISSN: 1792-1074
Online ISSN:1792-1082

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Jin S, Liu MD, Wu H, Pang P, Wang S, Li ZN, Sun CF and Liu FY: Overexpression of hsa‑miR‑125a‑5p enhances proliferation, migration and invasion of head and neck squamous cell carcinoma cell lines by upregulating C‑C chemokine receptor type 7. Oncol Lett 15: 9703-9710, 2018
APA
Jin, S., Liu, M., Wu, H., Pang, P., Wang, S., Li, Z. ... Liu, F. (2018). Overexpression of hsa‑miR‑125a‑5p enhances proliferation, migration and invasion of head and neck squamous cell carcinoma cell lines by upregulating C‑C chemokine receptor type 7. Oncology Letters, 15, 9703-9710. https://doi.org/10.3892/ol.2018.8564
MLA
Jin, S., Liu, M., Wu, H., Pang, P., Wang, S., Li, Z., Sun, C., Liu, F."Overexpression of hsa‑miR‑125a‑5p enhances proliferation, migration and invasion of head and neck squamous cell carcinoma cell lines by upregulating C‑C chemokine receptor type 7". Oncology Letters 15.6 (2018): 9703-9710.
Chicago
Jin, S., Liu, M., Wu, H., Pang, P., Wang, S., Li, Z., Sun, C., Liu, F."Overexpression of hsa‑miR‑125a‑5p enhances proliferation, migration and invasion of head and neck squamous cell carcinoma cell lines by upregulating C‑C chemokine receptor type 7". Oncology Letters 15, no. 6 (2018): 9703-9710. https://doi.org/10.3892/ol.2018.8564