Mitogen‑activated protein kinase inhibition enhances the antitumor effects of sporamin in human pancreatic cancer cells

  • Authors:
    • Cui‑Juan Qian
    • Yong‑Xiao Qi
    • Sheng Zhong
    • Ju‑Ping Zeng
    • Xiao‑Ying Chen
    • Jun Yao
  • View Affiliations

  • Published online on: May 18, 2018     https://doi.org/10.3892/ol.2018.8746
  • Pages: 1237-1242
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Abstract

Sporamin, a sweet potato tuber storage protein, is a Kunitz‑type trypsin inhibitor (TI) that has exhibited antitumor activity through poorly defined mechanisms in a number of types of tumor cells. The present study aimed to analyze the combined effects of sporamin and three mitogen‑activated protein kinase (MAPK) inhibitors, PD98059, SP600125 and SB203580, on the pancreatic cancer cell line, PANC‑1. Cell proliferation activity was assessed using a 3H‑thymidine incorporation assay, and cell viability was analyzed using an MTT assay. Apoptosis was assayed by flow cytometry and fluorescence microscopy. Protein expression levels in PANC‑1 cells were determined by western blotting. The results of this analysis demonstrated that sporamin induced a temporary increase in the phosphorylation of MAPKs, including phosphorylated extracellular signal regulated‑kinase 1/2, phosphorylated c‑Jun amino‑terminal protein kinase 1/2 and phosphorylated p38‑MAPK, in a concentration‑dependent manner. However, treatment with MAPK inhibitors promoted the inhibition of cell proliferation and viability, and the induction of apoptosis in sporamin‑treated PANC‑1 cells. In conclusion, the present study demonstrated that MAPK inhibition enhanced the antitumor activity of sporamin in PANC‑1 cells.
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July-2018
Volume 16 Issue 1

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Qian CJ, Qi YX, Zhong S, Zeng JP, Chen XY and Yao J: Mitogen‑activated protein kinase inhibition enhances the antitumor effects of sporamin in human pancreatic cancer cells. Oncol Lett 16: 1237-1242, 2018
APA
Qian, C., Qi, Y., Zhong, S., Zeng, J., Chen, X., & Yao, J. (2018). Mitogen‑activated protein kinase inhibition enhances the antitumor effects of sporamin in human pancreatic cancer cells. Oncology Letters, 16, 1237-1242. https://doi.org/10.3892/ol.2018.8746
MLA
Qian, C., Qi, Y., Zhong, S., Zeng, J., Chen, X., Yao, J."Mitogen‑activated protein kinase inhibition enhances the antitumor effects of sporamin in human pancreatic cancer cells". Oncology Letters 16.1 (2018): 1237-1242.
Chicago
Qian, C., Qi, Y., Zhong, S., Zeng, J., Chen, X., Yao, J."Mitogen‑activated protein kinase inhibition enhances the antitumor effects of sporamin in human pancreatic cancer cells". Oncology Letters 16, no. 1 (2018): 1237-1242. https://doi.org/10.3892/ol.2018.8746