Preliminary investigation of the function of hsa_circ_0006215 in pancreatic cancer

  • Authors:
    • Ping Zhu
    • Nan Ge
    • Dongyan Liu
    • Fan Yang
    • Kai Zhang
    • Jintao Guo
    • Xiang Liu
    • Sheng Wang
    • Guoxin Wang
    • Siyu Sun
  • View Affiliations

  • Published online on: May 7, 2018     https://doi.org/10.3892/ol.2018.8652
  • Pages: 603-611
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Abstract

The incidence of pancreatic cancer is increasing annually in Asia as a whole. Pancreatic cancer ranks sixth in terms of incidence of all malignant tumors. Circular RNA (circRNA) is a type of non‑coding RNA which forms a covalently closed continuous loop. CircRNA is extensively expressed in the cytoplasm, and is markedly conservative and stable. MicroRNA (miR)‑378a‑3p and human (hsa)_circ_0006215 were detected using the reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR) in tissue and cells. Western blot analysis detected the SERPINA4 and hsa_circ_0006215 expression in tissue. A Cell Counting Kit‑8 assay was used to determine cell stability. Flow cytometry was used to determine the cell apoptotic rate. Transwell assays were used to determine cell migration. hsa_circ_0006215 was identified as a significantly upregulated circRNA. RT‑qPCR results verified that, in 30 samples of pancreatic cancer tissue and paracancerous tissue, hsa_circ_0006215 expression was increased in pancreatic cancer tissue, miR‑378a‑3p expression was decreased in pancreatic cancer tissue, and SERPINA4 expression was increased in pancreatic cancer tissue (P<0.05). Using bioinformatics database and bioinformatics analysis, the interaction network of hsa_circ_0006215 indicated that this circRNA was most likely to regulate the expression of miR‑378a‑3p. Further interaction analysis revealed that the SERPINA4 gene was a regulatory target gene most likely to have an influence. The present study identified the effects of hsa_circ_0006215, miR‑378a‑3p and SERPINA4 signaling pathways in pancreatic cancer cells.
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July-2018
Volume 16 Issue 1

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Zhu P, Ge N, Liu D, Yang F, Zhang K, Guo J, Liu X, Wang S, Wang G, Sun S, Sun S, et al: Preliminary investigation of the function of hsa_circ_0006215 in pancreatic cancer. Oncol Lett 16: 603-611, 2018
APA
Zhu, P., Ge, N., Liu, D., Yang, F., Zhang, K., Guo, J. ... Sun, S. (2018). Preliminary investigation of the function of hsa_circ_0006215 in pancreatic cancer. Oncology Letters, 16, 603-611. https://doi.org/10.3892/ol.2018.8652
MLA
Zhu, P., Ge, N., Liu, D., Yang, F., Zhang, K., Guo, J., Liu, X., Wang, S., Wang, G., Sun, S."Preliminary investigation of the function of hsa_circ_0006215 in pancreatic cancer". Oncology Letters 16.1 (2018): 603-611.
Chicago
Zhu, P., Ge, N., Liu, D., Yang, F., Zhang, K., Guo, J., Liu, X., Wang, S., Wang, G., Sun, S."Preliminary investigation of the function of hsa_circ_0006215 in pancreatic cancer". Oncology Letters 16, no. 1 (2018): 603-611. https://doi.org/10.3892/ol.2018.8652