Open Access

miR‑885‑5p upregulation promotes colorectal cancer cell proliferation and migration by targeting suppressor of cytokine signaling

Retraction in: /10.3892/ol.2024.14333

  • Authors:
    • Meng Su
    • Baoli Qin
    • Fang Liu
    • Yuze Chen
    • Rui Zhang
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  • Published online on: May 7, 2018     https://doi.org/10.3892/ol.2018.8645
  • Pages: 65-72
  • Copyright: © Su et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The aim of the present study was to investigate the role of microRNA (miR)‑885‑5p in colorectal cancer cell proliferation and migration, and to determine the possible underlying molecular mechanisms. The expression of miR‑885‑5p in colorectal cancer tissue and cells was detected by reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR). The expression levels of three suppressor of cytokine signaling (SOCS) factors were detected by RT‑qPCR and western blotting. The effects of miR‑885‑5p on tumor cell proliferation and migration were studied using MTT and Transwell assays, respectively. Additionally, the expression levels of epithelial‑mesenchymal transition (EMT)‑related proteins (N‑cadherin, E‑cadherin, vimentin and Snail) were detected by RT‑qPCR and western blot analysis. Furthermore, the target of miR‑885‑5p was predicted and confirmed using a luciferase reporter assay. miR‑885‑5p was demonstrated to be upregulated and SOCS was downregulated in colorectal cancer tissue, and cells. miR‑885‑5p suppression significantly inhibited tumor cell proliferation and migration, promoted E‑cadherin expression, and inhibited the expression levels of N‑cadherin, vimentin and Snail. Further studies showed that SOCS5, SOCS6 and SOCS7 were direct targets of miR‑885‑5p. The results suggest that miR‑885‑5p suppression inhibited cell proliferation and migration, and the EMT process by targeting SOCS5, SOCS6 and SOCS7 genes in colorectal cancer. miR‑885‑5p and SOCS may be used for the diagnosis and treatment of colorectal cancer.
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July-2018
Volume 16 Issue 1

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Su M, Qin B, Liu F, Chen Y and Zhang R: miR‑885‑5p upregulation promotes colorectal cancer cell proliferation and migration by targeting suppressor of cytokine signaling Retraction in /10.3892/ol.2024.14333. Oncol Lett 16: 65-72, 2018
APA
Su, M., Qin, B., Liu, F., Chen, Y., & Zhang, R. (2018). miR‑885‑5p upregulation promotes colorectal cancer cell proliferation and migration by targeting suppressor of cytokine signaling Retraction in /10.3892/ol.2024.14333. Oncology Letters, 16, 65-72. https://doi.org/10.3892/ol.2018.8645
MLA
Su, M., Qin, B., Liu, F., Chen, Y., Zhang, R."miR‑885‑5p upregulation promotes colorectal cancer cell proliferation and migration by targeting suppressor of cytokine signaling Retraction in /10.3892/ol.2024.14333". Oncology Letters 16.1 (2018): 65-72.
Chicago
Su, M., Qin, B., Liu, F., Chen, Y., Zhang, R."miR‑885‑5p upregulation promotes colorectal cancer cell proliferation and migration by targeting suppressor of cytokine signaling Retraction in /10.3892/ol.2024.14333". Oncology Letters 16, no. 1 (2018): 65-72. https://doi.org/10.3892/ol.2018.8645