Open Access

Knockdown of B7H6 inhibits tumor progression in triple‑negative breast cancer

  • Authors:
    • Bing Zhang
    • Jinzhong Sun
    • Xiaoli Yao
    • Juanjuan Li
    • Yi Tu
    • Feng Yao
    • Shengrong Sun
  • View Affiliations

  • Published online on: May 10, 2018     https://doi.org/10.3892/ol.2018.8689
  • Pages: 91-96
  • Copyright: © Zhang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

The B7 family, the most common family of secondary signaling molecules, consists of eight cell‑surface proteins, which regulate the T‑cell mediated immune response by delivering co‑inhibitory or co‑stimulatory signals through their corresponding ligands. Among them, natural killer cell cytotoxicity receptor 3 ligand 1 (NCR3LG1, also known as B7H6) has been reported as a new member, and is involved in tumor progression of various types of human cancer. However, the role of B7H6 in triple‑negative breast cancer (TNBC) remains unknown. In the present study, western blotting was performed to determine the protein expression levels of B7H6 in a normal mammary epithelial cell line (MCF‑10A), non‑TNBC breast cancer cell lines (MCF‑7 and AU565) and TNBC cell lines (MDA‑MB‑231 and MDA‑MB‑468). B7H6 was knocked down using small interfering RNA, and an MTT assay was performed to determine proliferation ability, flow cytometry was used to analyze apoptosis, and Transwell and wound‑healing assays were performed to measure migration ability. Expression of proliferation‑associated proteins (SMAD family member 4 and β‑catenin) and apoptosis‑associated proteins (BCL2 associated X, BCL2 apoptosis regulator and caspase‑3) were analyzed by western blotting. The results demonstrated that B7H6 was highly expressed in TNBC cells, and that knockdown of B7H6 inhibited cell proliferation and migration, and promoted apoptosis. Furthermore, the results revealed that proliferation and apoptosis‑associated proteins were altered in the B7H6‑knockdown MDA‑MB‑231 cells. In conclusion, the present study demonstrated that B7H6 may have significant roles in the regulation of cell proliferation, apoptosis and migration of TNBC cells.
View Figures
View References

Related Articles

Journal Cover

July-2018
Volume 16 Issue 1

Print ISSN: 1792-1074
Online ISSN:1792-1082

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Zhang B, Sun J, Yao X, Li J, Tu Y, Yao F and Sun S: Knockdown of B7H6 inhibits tumor progression in triple‑negative breast cancer. Oncol Lett 16: 91-96, 2018
APA
Zhang, B., Sun, J., Yao, X., Li, J., Tu, Y., Yao, F., & Sun, S. (2018). Knockdown of B7H6 inhibits tumor progression in triple‑negative breast cancer. Oncology Letters, 16, 91-96. https://doi.org/10.3892/ol.2018.8689
MLA
Zhang, B., Sun, J., Yao, X., Li, J., Tu, Y., Yao, F., Sun, S."Knockdown of B7H6 inhibits tumor progression in triple‑negative breast cancer". Oncology Letters 16.1 (2018): 91-96.
Chicago
Zhang, B., Sun, J., Yao, X., Li, J., Tu, Y., Yao, F., Sun, S."Knockdown of B7H6 inhibits tumor progression in triple‑negative breast cancer". Oncology Letters 16, no. 1 (2018): 91-96. https://doi.org/10.3892/ol.2018.8689