Open Access

DEP domain containing 1 suppresses apoptosis via inhibition of A20 expression, which activates the nuclear factor κB signaling pathway in HepG2 cells

  • Authors:
    • Aili Li
    • Qingqing Wang
    • Gaofeng He
    • Junfei Jin
    • Guojin Huang
  • View Affiliations

  • Published online on: May 22, 2018     https://doi.org/10.3892/ol.2018.8770
  • Pages: 949-955
  • Copyright: © Li et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

A previous study revealed that DEP domain containing 1 (DEPDC1) is involved in the carcinogenesis of bladder cancer via forming a complex with zinc finger protein 224 (ZNF224) to suppress A20 expression, resulting in the activation of the nuclear factor (NF)‑κB signaling pathway; however, the role of DEPDC1 in liver cancer remains unclear. Hep G2 cells were treated with 11R‑DEP: 611‑628, a peptide capable of disrupting the DEPDC1‑ZNF224 complex. Cell proliferation was examined using an MTT assay and apoptosis was analyzed via detection of the apoptotic marker caspase‑3 using western blot analysis. A20 expression was examined via reverse transcription‑quantitative polymerase chain reaction and NF‑κB subcellular localization was determined via immunofluorescence staining. microRNA (miR)‑130a was overexpressed in HepG2 cells and its effects on proliferation and apoptosis were examined. The results demonstrated that 11R‑DEP: 611‑628 (3 µM) and miR‑130a inhibited cell proliferation and promoted apoptosis in HepG2 cells by activating A20 expression, which blocks the nuclear transportation of NF‑κB. In addition, the results demonstrated that the 11R‑DEP: 611‑628 (3 µM) treatment resulted in downregulation of DEPDC1 expression, indicating that DEPDC1 expression is regulated by the DEPDC1‑ZNF224 complex. In conclusion, the data indicated that DEPDC1 suppresses apoptosis to promote cell proliferation through the NF‑κB signaling pathway in HepG2 cells and that DEPDC1 is a potential target for the treatment of liver cancer.
View Figures
View References

Related Articles

Journal Cover

July-2018
Volume 16 Issue 1

Print ISSN: 1792-1074
Online ISSN:1792-1082

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Li A, Wang Q, He G, Jin J and Huang G: DEP domain containing 1 suppresses apoptosis via inhibition of A20 expression, which activates the nuclear factor κB signaling pathway in HepG2 cells. Oncol Lett 16: 949-955, 2018
APA
Li, A., Wang, Q., He, G., Jin, J., & Huang, G. (2018). DEP domain containing 1 suppresses apoptosis via inhibition of A20 expression, which activates the nuclear factor κB signaling pathway in HepG2 cells. Oncology Letters, 16, 949-955. https://doi.org/10.3892/ol.2018.8770
MLA
Li, A., Wang, Q., He, G., Jin, J., Huang, G."DEP domain containing 1 suppresses apoptosis via inhibition of A20 expression, which activates the nuclear factor κB signaling pathway in HepG2 cells". Oncology Letters 16.1 (2018): 949-955.
Chicago
Li, A., Wang, Q., He, G., Jin, J., Huang, G."DEP domain containing 1 suppresses apoptosis via inhibition of A20 expression, which activates the nuclear factor κB signaling pathway in HepG2 cells". Oncology Letters 16, no. 1 (2018): 949-955. https://doi.org/10.3892/ol.2018.8770