Open Access

Expression of WNT1 in ameloblastoma and its significance

  • Authors:
    • Guan‑Nan Wang
    • Ming Zhong
    • Yv Chen
    • Jia Ji
    • Xiu‑Qiu Gao
    • Tian‑Fu Wang
  • View Affiliations

  • Published online on: May 25, 2018     https://doi.org/10.3892/ol.2018.8820
  • Pages: 1507-1512
  • Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The present study aimed to measure the expression of WNT1 in ameloblastoma (AB). Immunohistochemistry was used to observe changes in WNT1 expression in 80 AB samples, 10 keratocystic odontogenic tumor (KCOT) samples and 10 normal oral mucosa (NOM) samples. Western blotting and reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR) were used to measure WNT1 protein and mRNA expression, respectively, in 30 AB samples, 5 KCOT samples, 5 NOM samples and 3 tooth germ samples. Ectopic cytoplasmic expression of WNT1 was detected in AB; 88.8% (71/80) of the samples were WNT1‑positive. The western blotting results demonstrated that compared with NOM (0.57±0.05), WNT1 expression was significantly higher in AB tissue (1.74±0.36, P<0.05), whereas it was not significantly different between AB and KCOT samples (0.80±0.06, P>0.05). RT‑qPCR revealed that the level of WNT1 gene expression in AB was increased 2.43‑fold compared with normal mucosa, and 1.77‑fold compared with tooth germ tissue. In conclusion, WNT1 protein and mRNA expression were increased in AB, and there was ectopic cytoplasmic expression. This indicates that WNT1 may serve an important role in AB occurrence and development.
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August-2018
Volume 16 Issue 2

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Copy and paste a formatted citation
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Spandidos Publications style
Wang GN, Zhong M, Chen Y, Ji J, Gao XQ and Wang TF: Expression of WNT1 in ameloblastoma and its significance. Oncol Lett 16: 1507-1512, 2018
APA
Wang, G., Zhong, M., Chen, Y., Ji, J., Gao, X., & Wang, T. (2018). Expression of WNT1 in ameloblastoma and its significance. Oncology Letters, 16, 1507-1512. https://doi.org/10.3892/ol.2018.8820
MLA
Wang, G., Zhong, M., Chen, Y., Ji, J., Gao, X., Wang, T."Expression of WNT1 in ameloblastoma and its significance". Oncology Letters 16.2 (2018): 1507-1512.
Chicago
Wang, G., Zhong, M., Chen, Y., Ji, J., Gao, X., Wang, T."Expression of WNT1 in ameloblastoma and its significance". Oncology Letters 16, no. 2 (2018): 1507-1512. https://doi.org/10.3892/ol.2018.8820