Open Access

Functional cardiac Na+ channels are expressed in human melanoma cells

  • Authors:
    • An Xie
    • Benjamin Gallant
    • Hao Guo
    • Alfredo Gonzalez
    • Matthew Clark
    • Audrey Madigan
    • Feng Feng
    • Hong‑Duo Chen
    • Yali Cui
    • Samuel C. Dudley
    • Yinsheng Wan
  • View Affiliations

  • Published online on: May 31, 2018     https://doi.org/10.3892/ol.2018.8865
  • Pages: 1689-1695
  • Copyright: © Xie et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Resting membrane potential (RMP) and intracellular Ca2+ concentration [(Ca2+)i] are involved in tumorigenesis and metastasis. The present study investigated whether functional cardiac Na+ channels are expressed in human melanoma cells (WM 266‑4) and its nonmalignant human melanocytes (HMC), as well as whether they participate in RMP maintenance and Ca2+ homeostasis. Confocal microscopy and western blot analysis were used to detect Na+ channels. The patch‑clamp technique was employed to record Na+ currents and action potentials. Cytoplasmic Ca2+ was measured by loading Fluo‑4. Cardiac (Nav1.5) Na+ channels were expressed in HMCs and WM 266‑4 cells. Tetrodotoxin (TTX) dose‑dependently blocked Na+ currents in WM 266‑4 while HMCs had no Na+ currents. Ultraviolet light induced similar action potentials in HMCs and WM 266‑4 cells, which were abolished by transient receptor potential A1 channel‑specific blocker, HC‑030031. Compared with HMCs, RMP was substantially depolarized in WM 266‑4. TTX hyperpolarized RMP in WM 266‑4 cells at a concentration of 30 µM, which facilitated Ca2+ influx. Compared with HMCs, (Ca2+)i was significantly higher in WM 266‑4 cells and was elevated by 30 µM TTX. Collectively, Cardiac Na+ channels depolarize RMP and inhibit Ca2+ uptake in melanoma cells possibly contributing to tumorigenesis and metastasis. Na+ channel agonists may be developed to treat melanoma such as WM 266‑4.
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August-2018
Volume 16 Issue 2

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Xie A, Gallant B, Guo H, Gonzalez A, Clark M, Madigan A, Feng F, Chen HD, Cui Y, Dudley SC, Dudley SC, et al: Functional cardiac Na+ channels are expressed in human melanoma cells. Oncol Lett 16: 1689-1695, 2018
APA
Xie, A., Gallant, B., Guo, H., Gonzalez, A., Clark, M., Madigan, A. ... Wan, Y. (2018). Functional cardiac Na+ channels are expressed in human melanoma cells. Oncology Letters, 16, 1689-1695. https://doi.org/10.3892/ol.2018.8865
MLA
Xie, A., Gallant, B., Guo, H., Gonzalez, A., Clark, M., Madigan, A., Feng, F., Chen, H., Cui, Y., Dudley, S. C., Wan, Y."Functional cardiac Na+ channels are expressed in human melanoma cells". Oncology Letters 16.2 (2018): 1689-1695.
Chicago
Xie, A., Gallant, B., Guo, H., Gonzalez, A., Clark, M., Madigan, A., Feng, F., Chen, H., Cui, Y., Dudley, S. C., Wan, Y."Functional cardiac Na+ channels are expressed in human melanoma cells". Oncology Letters 16, no. 2 (2018): 1689-1695. https://doi.org/10.3892/ol.2018.8865