Thapsigargin induces apoptosis of prostate cancer through cofilin‑1 and paxillin

  • Authors:
    • Fengyu Huang
    • Peitao Wang
    • Xinsheng Wang
  • View Affiliations

  • Published online on: May 30, 2018     https://doi.org/10.3892/ol.2018.8833
  • Pages: 1975-1980
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Abstract

It is widely considered that endoplasmic reticulum stress may rapidly induce apoptosis. The aim of the present study was to investigate the effect of thapsigargin on the induction of apoptosis in prostate cancer cells, and to explore its possible mechanism. A Cell Counting Kit‑8 was selected to determine the effect of thapsigargin (0, 1, 10 and 100 nM) on the proliferation of PC3 cells. Cell proliferation of the prostate cancer cells was effectively inhibited by treatment with thapsigargin, and thapsigargin significantly increased the rate of apoptosis and caspase‑3/9 activities in prostate cancer cells. The protein expression of phosphorylated (p)‑RAC‑α serine threonine‑protein kinase, p‑mechanistic target of rapamycin, F‑actin and paxillin were significantly decreased, and cofilin‑1 protein expression was significantly increased by treatment with thapsigargin in prostate cancer cells. Overall, the data of the present study revealed that thapsigargin induced apoptosis in prostate cancer cells through cofilin‑1 and paxillin.
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August-2018
Volume 16 Issue 2

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Huang F, Wang P and Wang X: Thapsigargin induces apoptosis of prostate cancer through cofilin‑1 and paxillin. Oncol Lett 16: 1975-1980, 2018
APA
Huang, F., Wang, P., & Wang, X. (2018). Thapsigargin induces apoptosis of prostate cancer through cofilin‑1 and paxillin. Oncology Letters, 16, 1975-1980. https://doi.org/10.3892/ol.2018.8833
MLA
Huang, F., Wang, P., Wang, X."Thapsigargin induces apoptosis of prostate cancer through cofilin‑1 and paxillin". Oncology Letters 16.2 (2018): 1975-1980.
Chicago
Huang, F., Wang, P., Wang, X."Thapsigargin induces apoptosis of prostate cancer through cofilin‑1 and paxillin". Oncology Letters 16, no. 2 (2018): 1975-1980. https://doi.org/10.3892/ol.2018.8833