Open Access

Decreased BMP‑7 and p‑Smad1/5/8 expression, and increased levels of gremlin in hepatocellular carcinoma

  • Authors:
    • Lina Wang
    • Qin Ding
    • Li Zhao
    • Yu Pan
    • Zhengxia Song
    • Yanan Qin
    • Xuebing Yan
  • View Affiliations

  • Published online on: June 6, 2018     https://doi.org/10.3892/ol.2018.8918
  • Pages: 2113-2118
  • Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The aim of the present study was to investigate the expression of bone morphogenetic protein‑7 (BMP‑7), gremlin, and p‑Smad/1/5/8 in carcinomatous and para‑carcinoma tissue specimens from patients with hepatocellular carcinoma (HCC). The association of serum BMP‑7 levels with clinicopathological parameters was examined to assess its relevance as a clinical biomarker for HCC. A total of 27 patients with HCC and 7 healthy subjects were included. Gene expression levels of BMP‑7 and p‑Smad1/5/8 were examined by reverse transcription‑quantitative polymerase chain reaction. Immunohistochemical and western blot analysis were performed to determine the protein expression of target genes. The serum levels of BMP‑7 were assessed by enzyme linked immunosorbent assay. The mRNA and protein expression of BMP‑7 and gremlin were significantly down‑ and upregulated in HCC tumor tissues, respectively, compared with para‑carcinoma tissues (P<0.05). The association of BMP‑7 and gremlin expression with the differentiation status of HCC was also analyzed. There was a relatively higher level of BMP‑7 and a lower level of gremlin expression in tumor tissues from patients with highly differentiated HCC when compared with poorly or moderately differentiated HCC (BMP‑7, F=42.29, P<0.01; gremlin, F=37.93, P<0.01). Furthermore, the level of BMP‑7 and p‑Smad1/5/8 was decreased in patients with advanced stages of HCC, when compared with stage I HCC. The findings from the present study suggest that the BMP‑7/p‑Smad signaling pathway may be involved in the pathogenesis of HCC. The serum levels of BMP‑7 may serve as a potential biomarker for HCC.
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August-2018
Volume 16 Issue 2

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Wang L, Ding Q, Zhao L, Pan Y, Song Z, Qin Y and Yan X: Decreased BMP‑7 and p‑Smad1/5/8 expression, and increased levels of gremlin in hepatocellular carcinoma. Oncol Lett 16: 2113-2118, 2018
APA
Wang, L., Ding, Q., Zhao, L., Pan, Y., Song, Z., Qin, Y., & Yan, X. (2018). Decreased BMP‑7 and p‑Smad1/5/8 expression, and increased levels of gremlin in hepatocellular carcinoma. Oncology Letters, 16, 2113-2118. https://doi.org/10.3892/ol.2018.8918
MLA
Wang, L., Ding, Q., Zhao, L., Pan, Y., Song, Z., Qin, Y., Yan, X."Decreased BMP‑7 and p‑Smad1/5/8 expression, and increased levels of gremlin in hepatocellular carcinoma". Oncology Letters 16.2 (2018): 2113-2118.
Chicago
Wang, L., Ding, Q., Zhao, L., Pan, Y., Song, Z., Qin, Y., Yan, X."Decreased BMP‑7 and p‑Smad1/5/8 expression, and increased levels of gremlin in hepatocellular carcinoma". Oncology Letters 16, no. 2 (2018): 2113-2118. https://doi.org/10.3892/ol.2018.8918