Open Access

Effects of miRNA-130a on the proliferation and apoptosis of glioma cell lines

  • Authors:
    • Ke Lu
    • Hechun Shen
    • Sheng Zhu
    • Shuiqing Bi
    • Shengtian Wu
  • View Affiliations

  • Published online on: June 1, 2018     https://doi.org/10.3892/ol.2018.8878
  • Pages: 2478-2482
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Abstract

Regulatory ability of micro-ribose nucleic acid‑130a (miRNA-130a) in the proliferation and invasive growth of human brain glioma cells and its mechanism were investigated. RT-qPCR was used to analyze expression of miRNA-130a in U-87MG glioma specimens; lipidosome was used to mediate miRNA‑130a mimic transfecting glioma cells and the expression of miRNA‑130a was detected by using RT-qPCR after transfection; methyl thiazolyl tetrazolium (MTT) assay and flow cytometry (FCM) were adopted to evaluate the changes in biological characteristics of cell growth and proliferation; the migration and invasion abilities of tumor cells were measured through scratch assay and Transwell in vitro cell migration assay. In miRNA-130a mimic-transfected U-87MG cells, RT-qPCR showed that the expression of miRNA-130a was upregulated; MTT assay and FCM revealed that the cell growth was strengthened; scratch assay and Transwell in vitro cell migration assay verified that the migration and invasion abilities of cells were enhanced. In conclusion, the high expression of miRNA-130a can promote growth and invasion, indicating that miRNA-130a can be considered as a candidate target of gene therapy for glioma.
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August-2018
Volume 16 Issue 2

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Lu K, Shen H, Zhu S, Bi S and Wu S: Effects of miRNA-130a on the proliferation and apoptosis of glioma cell lines. Oncol Lett 16: 2478-2482, 2018
APA
Lu, K., Shen, H., Zhu, S., Bi, S., & Wu, S. (2018). Effects of miRNA-130a on the proliferation and apoptosis of glioma cell lines. Oncology Letters, 16, 2478-2482. https://doi.org/10.3892/ol.2018.8878
MLA
Lu, K., Shen, H., Zhu, S., Bi, S., Wu, S."Effects of miRNA-130a on the proliferation and apoptosis of glioma cell lines". Oncology Letters 16.2 (2018): 2478-2482.
Chicago
Lu, K., Shen, H., Zhu, S., Bi, S., Wu, S."Effects of miRNA-130a on the proliferation and apoptosis of glioma cell lines". Oncology Letters 16, no. 2 (2018): 2478-2482. https://doi.org/10.3892/ol.2018.8878