Expression of galectin‑7 in vulvar lichen sclerosus and its effect on dermal fibroblasts

  • Authors:
    • Yi Zhao
    • Shanshan Zhao
    • Hui Li
    • Xin Qin
    • Xin Wu
  • View Affiliations

  • Published online on: June 4, 2018     https://doi.org/10.3892/ol.2018.8897
  • Pages: 2559-2564
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Abstract

Lichen sclerosus is a chronic and inflammatory disease. Extensive studies have focused on the epidermis, with the dermis or epidermis‑dermis receiving less attention. To investigate the role of galectin‑7, a keratinocyte protein, in vulvar lichen sclerosus (VLS) and its potential effects on dermal fibroblasts, immunohistochemical staining was performed with VLS tissue samples and normal control samples. The expression of galectin‑7 was determined by evaluating the galectin‑7 integrated density analysis, and further assessed by western blot analysis. Dermal fibroblasts were isolated from the normal tissue of the female anogenital region following sexual plastic surgery. A cell viability assay was performed on isolated dermal fibroblast cells in the presence or absence of galectin‑7. Reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR) was performed to determine the transcriptional level of collagen I and collagen III in the response to different doses of galectin‑7. In the immunohistochemical analysis, galectin‑7 demonstrated a significantly elevated level in VLS, compared to control tissues, which was confirmed by western blot analysis. In the analysis of primary dermal fibroblast cells, galectin‑7 significantly inhibited the viability rate of fibroblasts in a dose‑dependent manner. RT‑qPCR data revealed that the transcription level of collagen I and collagen III were positively associated with the galectin‑7 treatment concentration. The overexpression of galectin‑7 is associated with the progression of VLS in the epidermis, a high concentration of galectin‑7 inhibits the viability of the primary vulvar dermal fibroblasts, and stimulates the accumulation of collagen I and collagen III in dermal fibroblast cultures, thus galectin‑7 may serve as a drug target during VLS progression.
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August-2018
Volume 16 Issue 2

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Zhao Y, Zhao S, Li H, Qin X and Wu X: Expression of galectin‑7 in vulvar lichen sclerosus and its effect on dermal fibroblasts. Oncol Lett 16: 2559-2564, 2018
APA
Zhao, Y., Zhao, S., Li, H., Qin, X., & Wu, X. (2018). Expression of galectin‑7 in vulvar lichen sclerosus and its effect on dermal fibroblasts. Oncology Letters, 16, 2559-2564. https://doi.org/10.3892/ol.2018.8897
MLA
Zhao, Y., Zhao, S., Li, H., Qin, X., Wu, X."Expression of galectin‑7 in vulvar lichen sclerosus and its effect on dermal fibroblasts". Oncology Letters 16.2 (2018): 2559-2564.
Chicago
Zhao, Y., Zhao, S., Li, H., Qin, X., Wu, X."Expression of galectin‑7 in vulvar lichen sclerosus and its effect on dermal fibroblasts". Oncology Letters 16, no. 2 (2018): 2559-2564. https://doi.org/10.3892/ol.2018.8897