Open Access

Downregulation of LPXN expression by siRNA decreases the malignant proliferation and transmembrane invasion of SHI‑1 cells

  • Authors:
    • Guo‑Hua Zhu
    • Hai‑Ping Dai
    • Qun Shen
    • Qi Zhang
  • View Affiliations

  • Published online on: October 22, 2018     https://doi.org/10.3892/ol.2018.9605
  • Pages: 135-140
  • Copyright: © Zhu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

The aim of the present study was to investigate the effects of decreasing leupaxin (LPXN) expression on the proliferation and invasion of human acute monocytic leukemia SHI‑1 cells. The transfection efficiency of fluorescein amidite (FAM)‑small interfering RNA (siRNA) was determined using flow cytometry, and the protein expression levels of LPXN, phosphorylated (p)‑c‑Jun N‑terminal kinase (JNK), p‑p38 mitogen‑activated protein kinase (p38 MAPK) and p‑extracellular‑signal‑regulated kinase (ERK) were detected by western blot analysis. Proliferation was determined using the cell counting kit‑8 reagent and cellular transmembrane invasion ability was determined using a Transwell chamber system. The gelatinase levels of matrix metalloproteinase (MMP)‑2 and MMP‑9 in the cell culture supernatant were also analyzed by gelatin zymography. In SHI‑1 cells, the optimal transfection conditions of siRNA were a cell density of 4x105 cells/ml and a ratio of siRNA/Lipofectamine® 2000 of 200 pmol/1 µl. The highest transfection efficiency of FAM‑siRNA was 74.5%. In the present study, L2‑siRNA was selected to effectively decrease the expression of LPXN. Following downregulation of LPXN expression by L2‑siRNA, proliferation inhibition rates increased to 27.043±2.051 and cell transmembrane invasion rates decreased to 25.270±2.145 (P<0.05). The results of the western blot analysis and the gelatin zymography indicated that downregulation of LPXN expression increased the expression of p‑p38 MAPK and p‑JNK, and attenuated the secretion levels of MMP‑2 and MMP‑9. However, downregulation of LPXN expression had no effect on p‑ERK expression in SHI‑1 cells. The results of the present study indicated that downregulation of LPXN expression decreased the malignant proliferation and transmembrane invasion of SHI‑1 cells by activating JNK and p38 MAPK, and inhibiting MMP‑2 and MMP‑9 secretion.
View Figures
View References

Related Articles

Journal Cover

January-2019
Volume 17 Issue 1

Print ISSN: 1792-1074
Online ISSN:1792-1082

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Zhu GH, Dai HP, Shen Q and Zhang Q: Downregulation of LPXN expression by siRNA decreases the malignant proliferation and transmembrane invasion of SHI‑1 cells. Oncol Lett 17: 135-140, 2019
APA
Zhu, G., Dai, H., Shen, Q., & Zhang, Q. (2019). Downregulation of LPXN expression by siRNA decreases the malignant proliferation and transmembrane invasion of SHI‑1 cells. Oncology Letters, 17, 135-140. https://doi.org/10.3892/ol.2018.9605
MLA
Zhu, G., Dai, H., Shen, Q., Zhang, Q."Downregulation of LPXN expression by siRNA decreases the malignant proliferation and transmembrane invasion of SHI‑1 cells". Oncology Letters 17.1 (2019): 135-140.
Chicago
Zhu, G., Dai, H., Shen, Q., Zhang, Q."Downregulation of LPXN expression by siRNA decreases the malignant proliferation and transmembrane invasion of SHI‑1 cells". Oncology Letters 17, no. 1 (2019): 135-140. https://doi.org/10.3892/ol.2018.9605