Open Access

Identification of plasma RGS18 and PPBP mRNAs as potential biomarkers for gastric cancer using transcriptome arrays

  • Authors:
    • Chen Su
    • Hanwei Li
    • Zheng Peng
    • Dong Ke
    • Hanjiang Fu
    • Xiaofei Zheng
  • View Affiliations

  • Published online on: October 23, 2018     https://doi.org/10.3892/ol.2018.9608
  • Pages: 247-255
  • Copyright: © Su et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Coding and noncoding RNAs serve a crucial role in tumorigenesis. Circulating RNAs have been recognized as a novel category of biomarkers for a variety of physiological and pathological conditions. To identify plasma RNA biomarkers for gastric cancer (GC), a genome‑wide transcriptome analysis using GeneChip® Human Transcriptome Array, which contains probe sets covering exons of ~67500 coding and noncoding transcripts of annotated genes, was performed to screen for the RNAs that exhibited differential expression in the plasma samples of patients with GC and controls. The expression levels of 6 candidate RNAs, including regulator of G‑protein signaling 18 (RGS18), integral membrane protein 2B, pro‑platelet basic protein (PPBP), nucleosome assembly protein1‑like 1, n324674 and ENST00000442382 were assessed in the plasma samples of 81 patients with GC and 77 healthy participants using reverse transcription‑quantitative polymerase chain reaction. Furthermore, the expression levels of RGS18 and PPBP mRNAs were indicated to be significantly differentially expressed (P<0.0001) in an independent panel of plasma samples of 36 patients with GC compared with 34 healthy participants. The potential association of RGS18 and PPBP mRNA expression levels with clinicopathological features was subsequently analyzed. Receiver operating characteristic analysis indicated that the combination of these 2 mRNAs with an area under curve <0.812 was an improved indicator for gastric cancer compared with respective individual levels. The results of the present study indicate that RGS18 and PPBP mRNA expression was significantly downregulated in the plasma of patients with GC, and the combination of these 2 mRNAs may be a useful diagnostic or prognostic marker for GC.
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January-2019
Volume 17 Issue 1

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Spandidos Publications style
Su C, Li H, Peng Z, Ke D, Fu H and Zheng X: Identification of plasma RGS18 and PPBP mRNAs as potential biomarkers for gastric cancer using transcriptome arrays. Oncol Lett 17: 247-255, 2019
APA
Su, C., Li, H., Peng, Z., Ke, D., Fu, H., & Zheng, X. (2019). Identification of plasma RGS18 and PPBP mRNAs as potential biomarkers for gastric cancer using transcriptome arrays. Oncology Letters, 17, 247-255. https://doi.org/10.3892/ol.2018.9608
MLA
Su, C., Li, H., Peng, Z., Ke, D., Fu, H., Zheng, X."Identification of plasma RGS18 and PPBP mRNAs as potential biomarkers for gastric cancer using transcriptome arrays". Oncology Letters 17.1 (2019): 247-255.
Chicago
Su, C., Li, H., Peng, Z., Ke, D., Fu, H., Zheng, X."Identification of plasma RGS18 and PPBP mRNAs as potential biomarkers for gastric cancer using transcriptome arrays". Oncology Letters 17, no. 1 (2019): 247-255. https://doi.org/10.3892/ol.2018.9608