Open Access

Knockdown of DNA/RNA‑binding protein KIN17 promotes apoptosis of triple‑negative breast cancer cells

  • Authors:
    • Xiang Gao
    • Zhenping Liu
    • Meifeng Zhong
    • Kunhe Wu
    • Yuzhao Zhang
    • Hongmei Wang
    • Tao Zeng
  • View Affiliations

  • Published online on: October 18, 2018     https://doi.org/10.3892/ol.2018.9597
  • Pages: 288-293
  • Copyright: © Gao et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Effective therapy for breast cancer has been extensively studied worldwide, particularly for triple‑negative breast cancer and drug‑resistant subtypes. DNA/RNA‑binding protein KIN17 (kin17) has been reported to be significantly upregulated in breast cancer cells, and is proposed to serve a role in the regulation of cell proliferation. The present study further investigated the association of kin17‑knockdown with breast cancer cell apoptosis. Cell Counting kit‑8, flow cytometry, TUNEL assay and caspase 3/7 analysis were performed on MDA‑MB‑231 cells to determine the association between kin17 and breast cancer cell apoptosis. In addition, western blot analysis was performed to investigate the mechanism of kin17 in the apoptosis of MDA‑MB‑231 cells. The results revealed that knockdown of kin17 inhibited proliferation and promoted apoptosis of MDA‑MB‑231 cells, and suggested a poly (adenosine diphosphate‑ribose) polymerase‑related mechanism behind the apoptosis of the cells. These findings suggested that kin17 could become a novel target for breast cancer therapy.
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January-2019
Volume 17 Issue 1

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Gao X, Liu Z, Zhong M, Wu K, Zhang Y, Wang H and Zeng T: Knockdown of DNA/RNA‑binding protein KIN17 promotes apoptosis of triple‑negative breast cancer cells. Oncol Lett 17: 288-293, 2019
APA
Gao, X., Liu, Z., Zhong, M., Wu, K., Zhang, Y., Wang, H., & Zeng, T. (2019). Knockdown of DNA/RNA‑binding protein KIN17 promotes apoptosis of triple‑negative breast cancer cells. Oncology Letters, 17, 288-293. https://doi.org/10.3892/ol.2018.9597
MLA
Gao, X., Liu, Z., Zhong, M., Wu, K., Zhang, Y., Wang, H., Zeng, T."Knockdown of DNA/RNA‑binding protein KIN17 promotes apoptosis of triple‑negative breast cancer cells". Oncology Letters 17.1 (2019): 288-293.
Chicago
Gao, X., Liu, Z., Zhong, M., Wu, K., Zhang, Y., Wang, H., Zeng, T."Knockdown of DNA/RNA‑binding protein KIN17 promotes apoptosis of triple‑negative breast cancer cells". Oncology Letters 17, no. 1 (2019): 288-293. https://doi.org/10.3892/ol.2018.9597