Open Access

Knockdown of CHCHD2 inhibits migration and angiogenesis of human renal cell carcinoma: A potential molecular marker for treatment of RCC

  • Authors:
    • Qian Cheng
    • Debao Qu
    • Zheng Lu
    • Longzhen Zhang
  • View Affiliations

  • Published online on: November 12, 2018     https://doi.org/10.3892/ol.2018.9686
  • Pages: 765-772
  • Copyright: © Cheng et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Coiled‑coil‑helix‑coiled‑coil‑helix domain‑­containing protein 2 (CHCHD2), a novel cell migration determinant, is able to co‑express with other genes of the oxidative phosphorylation pathway by using a computational expression screening technique. However, little is known about the expression and biological function of CHCHD2 in human renal cell carcinoma (RCC). Western blotting was performed to detect CHCHD2 expression levels in normal renal cells and carcinoma cells. Immunohistochemistry was performed to detect an association between CHCHD2 expression and clinicopathological parameters in 75 RCC tissues using a tissue microarray. The function of CHCHD2 in the migration and angiogenesis of RCC cells was investigated using Transwell migration and tube formation assays. CHCHD2 expression was markedly increased in human RCC cells. The results of immunohistochemical analysis revealed that CHCHD2 expression was markedly associated with tumor grade (P<0.001). Notably, CHCHD2 knockdown inhibited RCC migration and tube formation of human umbilical vascular endothelial cells. CHCHD2 knockdown further suppressed matrix metalloproteinase‑2 protein levels and enzyme activity. An ELISA identified that CHCHD2 knockdown decreased the secretion of vascular endothelial growth factor. The gathered data disclose information on the association of CHCHD2 with migration and angiogenesis of human RCC, and may strengthen the feasibility of targeting CHCHD2 as a potential therapeutic target.
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January-2019
Volume 17 Issue 1

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Cheng Q, Qu D, Lu Z and Zhang L: Knockdown of CHCHD2 inhibits migration and angiogenesis of human renal cell carcinoma: A potential molecular marker for treatment of RCC. Oncol Lett 17: 765-772, 2019
APA
Cheng, Q., Qu, D., Lu, Z., & Zhang, L. (2019). Knockdown of CHCHD2 inhibits migration and angiogenesis of human renal cell carcinoma: A potential molecular marker for treatment of RCC. Oncology Letters, 17, 765-772. https://doi.org/10.3892/ol.2018.9686
MLA
Cheng, Q., Qu, D., Lu, Z., Zhang, L."Knockdown of CHCHD2 inhibits migration and angiogenesis of human renal cell carcinoma: A potential molecular marker for treatment of RCC". Oncology Letters 17.1 (2019): 765-772.
Chicago
Cheng, Q., Qu, D., Lu, Z., Zhang, L."Knockdown of CHCHD2 inhibits migration and angiogenesis of human renal cell carcinoma: A potential molecular marker for treatment of RCC". Oncology Letters 17, no. 1 (2019): 765-772. https://doi.org/10.3892/ol.2018.9686