Open Access

MicroRNA‑885‑5p promotes osteosarcoma proliferation and migration by downregulation of cell division cycle protein 73 homolog expression

  • Authors:
    • Feng‑Qiang Yu
    • Zeng Wang
    • Xin‑Wen Wang
    • Sheng‑Lin Wang
    • Xiao‑Dong Li
    • Qing‑Shan Huang
    • Jian‑Hua Lin
  • View Affiliations

  • Published online on: December 6, 2018     https://doi.org/10.3892/ol.2018.9802
  • Pages: 1565-1572
  • Copyright: © Yu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Osteosarcoma (OS) is the most common primary malignant bone tumor. Numerous studies have strongly implicated the ectopic expression of microRNAs (miRNAs/miRs), including miR‑885‑5p, which is aberrantly expressed in several cancer types, in multiple cancer‑related processes. However, the role of miR‑885‑5p in OS remains unknown. In the present study, it was found that the expression of miR‑885‑5p was markedly upregulated in OS cell lines and clinical tissues. Moreover, high expression of miR‑885‑5p was significantly associated with the development of OS. The human OS MG‑63 cell line was transfected with recombinant lentivirus to regulate miR‑885‑5p expression. Overexpressed miR‑885‑5p significantly promoted the proliferation and migration of MG‑63 cells in vitro, while downregulating miR‑885‑5p expression reversed these effects. Furthermore, bioinformatic analysis was used to predict the potential target genes of miR‑885‑5p, and cell division cycle protein 73 homolog (CDC73) was identified as a novel and direct target of miR‑885‑5p. This interaction was further confirmed using reverse transcription‑quantitative polymerase chain reaction, western blotting and luciferase activity assays. These findings suggest that miR‑885‑5p serves a critical role in facilitating OS proliferation and migration, and can regulate CDC73 expression in OS cells and tissues. Thus, miR‑885‑5p could be a promising novel therapeutic biomarker for OS.
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February-2019
Volume 17 Issue 2

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Yu FQ, Wang Z, Wang XW, Wang SL, Li XD, Huang QS and Lin JH: MicroRNA‑885‑5p promotes osteosarcoma proliferation and migration by downregulation of cell division cycle protein 73 homolog expression. Oncol Lett 17: 1565-1572, 2019
APA
Yu, F., Wang, Z., Wang, X., Wang, S., Li, X., Huang, Q., & Lin, J. (2019). MicroRNA‑885‑5p promotes osteosarcoma proliferation and migration by downregulation of cell division cycle protein 73 homolog expression. Oncology Letters, 17, 1565-1572. https://doi.org/10.3892/ol.2018.9802
MLA
Yu, F., Wang, Z., Wang, X., Wang, S., Li, X., Huang, Q., Lin, J."MicroRNA‑885‑5p promotes osteosarcoma proliferation and migration by downregulation of cell division cycle protein 73 homolog expression". Oncology Letters 17.2 (2019): 1565-1572.
Chicago
Yu, F., Wang, Z., Wang, X., Wang, S., Li, X., Huang, Q., Lin, J."MicroRNA‑885‑5p promotes osteosarcoma proliferation and migration by downregulation of cell division cycle protein 73 homolog expression". Oncology Letters 17, no. 2 (2019): 1565-1572. https://doi.org/10.3892/ol.2018.9802