Open Access

MicroRNA‑504 modulates osteosarcoma cell chemoresistance to cisplatin by targeting p53

  • Authors:
    • Xin Chen
    • Chen Lv
    • Xiongbai Zhu
    • Wenjun Lin
    • Lu Wang
    • Zhengxiang Huang
    • Shengwu Yang
    • Junying Sun
  • View Affiliations

  • Published online on: November 22, 2018     https://doi.org/10.3892/ol.2018.9749
  • Pages: 1664-1674
  • Copyright: © Chen et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Chemoresistance implicates the therapeutic value of cisplatin and remains a primary obstacle to its clinical use. MicroRNAs (miRs) negatively modulate the expression of their target genes and are associated with the occurrence and progression of various types of tumor. The abnormal expression of miR‑504 has been reported in certain types of human tumor and has been associated with tumor prognosis. However, the association between miR‑504 and cisplatin in human osteosarcoma remains unclear. The present study therefore aimed to assess the in vitro effects and possible mechanism of miR‑504 in cell proliferation, apoptosis and cisplatin resistance in MG63 osteosarcoma cells. The results demonstrated that miR‑504 was overexpressed in osteosarcoma tissues and cells. This overexpression also induced cell proliferation, as determined by MTT and EdU staining assays. Furthermore, miR‑504 suppressed cisplatin‑induced apoptosis, which was demonstrated via MTT, cell morphology analysis and flow cytometry. Cisplatin‑induced G1 arrest was also suppressed, which was determined by flow cytometry. The potential target genes of miR‑504 were predicted using bioinformatics. p53 was confirmed to be a direct target of miR‑504 using a luciferase reporter assay and western blot analysis revealed that miR‑504 negatively regulated p53 expression at a molecular level. These results indicate that miR‑504 contributes to cisplatin resistance in MG63 osteosarcoma cells by suppressing p53. miR‑504 may therefore be a potential biomarker for cisplatin resistance in patients with osteosarcoma.
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February-2019
Volume 17 Issue 2

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Copy and paste a formatted citation
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Spandidos Publications style
Chen X, Lv C, Zhu X, Lin W, Wang L, Huang Z, Yang S and Sun J: MicroRNA‑504 modulates osteosarcoma cell chemoresistance to cisplatin by targeting p53. Oncol Lett 17: 1664-1674, 2019
APA
Chen, X., Lv, C., Zhu, X., Lin, W., Wang, L., Huang, Z. ... Sun, J. (2019). MicroRNA‑504 modulates osteosarcoma cell chemoresistance to cisplatin by targeting p53. Oncology Letters, 17, 1664-1674. https://doi.org/10.3892/ol.2018.9749
MLA
Chen, X., Lv, C., Zhu, X., Lin, W., Wang, L., Huang, Z., Yang, S., Sun, J."MicroRNA‑504 modulates osteosarcoma cell chemoresistance to cisplatin by targeting p53". Oncology Letters 17.2 (2019): 1664-1674.
Chicago
Chen, X., Lv, C., Zhu, X., Lin, W., Wang, L., Huang, Z., Yang, S., Sun, J."MicroRNA‑504 modulates osteosarcoma cell chemoresistance to cisplatin by targeting p53". Oncology Letters 17, no. 2 (2019): 1664-1674. https://doi.org/10.3892/ol.2018.9749