JTC‑801 inhibits the proliferation and metastasis of the Hep G2 hepatoblastoma cell line by regulating the phosphatidylinositol 3‑kinase/protein kinase B signalling pathway

  • Authors:
    • Bufei Zhao
    • Ting Hu
  • View Affiliations

  • Published online on: November 30, 2018     https://doi.org/10.3892/ol.2018.9780
  • Pages: 1939-1945
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

The increased worldwide mortality rate due to liver cancer may be attributed to the aggressive nature of the disease. Signal transduction through G‑protein‑coupled receptors (GPCRs) can affect a number of aspects of cancer biology, including invasion, migration and vascular remodelling. JTC‑801, a novel GPCR antagonist, has demonstrated promising anticancer effects in adenocarcinoma and osteosarcoma cells. In the present study, the effect of JTC‑801 on the proliferation and migration of hepatoblastoma Hep G2 cells was investigated. The Cell Counting Kit‑8 assay revealed that JTC‑801 markedly suppressed the growth of the Hep G2 cells. Additionally, JTC‑801 significantly inhibited cell invasion and migration in a Transwell assay. Furthermore, the expression of anti‑apoptotic protein B‑cell lymphoma 2 decreased and the expression of the pro‑apoptotic proteins active caspase‑3 and apoptosis regulator BAX increased in the Hep G2 cells following JTC‑801 treatment. Additionally, JTC‑801 suppressed the phosphatidylinositol 3‑kinase (PI3K)/protein kinase B (AKT) signalling pathway in the Hep G2 cells. Therefore, the present study revealed that JTC‑801 can induce the apoptosis of Hep G2 cells by regulating the PI3K/AKT signalling pathway, which suggests that JTC‑801 may be a potential novel drug target for clinical liver cancer treatment.
View Figures
View References

Related Articles

Journal Cover

February-2019
Volume 17 Issue 2

Print ISSN: 1792-1074
Online ISSN:1792-1082

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Zhao B and Zhao B: JTC‑801 inhibits the proliferation and metastasis of the Hep G2 hepatoblastoma cell line by regulating the phosphatidylinositol 3‑kinase/protein kinase B signalling pathway. Oncol Lett 17: 1939-1945, 2019
APA
Zhao, B., & Zhao, B. (2019). JTC‑801 inhibits the proliferation and metastasis of the Hep G2 hepatoblastoma cell line by regulating the phosphatidylinositol 3‑kinase/protein kinase B signalling pathway. Oncology Letters, 17, 1939-1945. https://doi.org/10.3892/ol.2018.9780
MLA
Zhao, B., Hu, T."JTC‑801 inhibits the proliferation and metastasis of the Hep G2 hepatoblastoma cell line by regulating the phosphatidylinositol 3‑kinase/protein kinase B signalling pathway". Oncology Letters 17.2 (2019): 1939-1945.
Chicago
Zhao, B., Hu, T."JTC‑801 inhibits the proliferation and metastasis of the Hep G2 hepatoblastoma cell line by regulating the phosphatidylinositol 3‑kinase/protein kinase B signalling pathway". Oncology Letters 17, no. 2 (2019): 1939-1945. https://doi.org/10.3892/ol.2018.9780