Open Access

miR-21 promotes cell migration and invasion of hepatocellular carcinoma by targeting KLF5

  • Authors:
    • Jian Wang
    • Yanfeng Chu
    • Mei Xu
    • Xiumei Zhang
    • Yumei Zhou
  • View Affiliations

  • Published online on: December 18, 2018     https://doi.org/10.3892/ol.2018.9843
  • Pages: 2221-2227
  • Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Previous studies have reported that microRNAs regulate gene expression and transcription. miR-21 have been identified to play a role in many types of cancer. KLF5 functions as a tumor inhibitor in certain cancers. However, the role of KLF5 plays in hepatocellular carcinoma (HCC), especially concerning the relationship between miR-21 and the KLF5 gene remains to be determined. Reverse transcription-quantitative PCR (RT-qPCR), western blot analysis, as well as luciferase reporter and Transwell assays were used to determine the expression of miR-21 and KLF5 in Huh 7, SK-HEP-1, LO-2, and HCC tissues. In HCC cells and tissues, the upregulation of miR-21 was identified. HCC cell migratory and invasive abilities significantly increased because of miR-21 over­expression. KLF5 expression was inhibited by miR-21 by targeting its 3'-UTR. KLF5 overexpression alleviated the effect induced by miR-21 on the migratory and invasive ability of the Huh 7 cells. The results therefore show that, HCC cell migration and invasion is significantly suppressed by miR-21 via targeting KLF5. The newly identified miR-21/KLF5 axis provides a useful therapeutic biomarker for HCC treatment.
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February-2019
Volume 17 Issue 2

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Copy and paste a formatted citation
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Spandidos Publications style
Wang J, Chu Y, Xu M, Zhang X and Zhou Y: miR-21 promotes cell migration and invasion of hepatocellular carcinoma by targeting KLF5. Oncol Lett 17: 2221-2227, 2019
APA
Wang, J., Chu, Y., Xu, M., Zhang, X., & Zhou, Y. (2019). miR-21 promotes cell migration and invasion of hepatocellular carcinoma by targeting KLF5. Oncology Letters, 17, 2221-2227. https://doi.org/10.3892/ol.2018.9843
MLA
Wang, J., Chu, Y., Xu, M., Zhang, X., Zhou, Y."miR-21 promotes cell migration and invasion of hepatocellular carcinoma by targeting KLF5". Oncology Letters 17.2 (2019): 2221-2227.
Chicago
Wang, J., Chu, Y., Xu, M., Zhang, X., Zhou, Y."miR-21 promotes cell migration and invasion of hepatocellular carcinoma by targeting KLF5". Oncology Letters 17, no. 2 (2019): 2221-2227. https://doi.org/10.3892/ol.2018.9843