Open Access

MicroRNA‑155 affects oxidative damage through regulating autophagy in endothelial cells

  • Authors:
    • Huifen Chen
    • Mi Yang Liu Gao
    • Li Zhang
    • Fa Lian He
    • Yan Kun Shi
    • Xing Hua Pan
    • Hong Wang
  • View Affiliations

  • Published online on: December 21, 2018     https://doi.org/10.3892/ol.2018.9860
  • Pages: 2237-2243
  • Copyright: © Chen et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

MicroRNA‑155 (miRNA‑155) is a typical multifunctional miRNA, which serves a crucial role in the regulation of numerous vessel cells. However, its effects on dysfunctional endothelial cells have not been completely elucidated. In order to investigate the signaling pathway of miRNA‑155‑induced cell injury, H2O2 was used to establish an oxidative stress cell model, and miR‑155 was transfected into H2O2‑treated cells. The CCK8 assay was then employed to examine the effect of miR‑155 on the cell proliferations of H2O2‑treated cells, and the expressions of Microtubule Associated Protein 1 Light Chain 3 (LC3) and Sequestosome 1 (P62) were detected to examine the effect of miR‑155 on the autophagy of Human umbilical vein endothelial cells, and then the formation of intracellular autophagosomes was observed. The results indicated that endothelial cell proliferation was promoted, and oxidant‑induced injury was decreased when the expression of miR‑155 was inhibited. In addition, the results also demonstrated that when the miR‑155 inhibitor was used, the expression of LC3 was increased and the expression of P62 was decreased. This suggests that modulated miR‑155 can prevent oxidative damage in endothelial cells, by regulating the level of autophagy. Furthermore, the present study also demonstrated that miR‑155 regulated autophagy via promotion of the expression of the autophagy‑related gene, Autophagy Related 5 (ATG5). In conclusion, the attenuated expression of miR‑155 can decrease oxidant‑induced injury and promote cell proliferation via upregulating autophagy, which subsequently affects the expression of ATG5. The present study provides a novel insight into microRNAs as potential therapeutics for the treatment of heart disease.
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February-2019
Volume 17 Issue 2

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Copy and paste a formatted citation
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Spandidos Publications style
Chen H, Liu Gao MY, Zhang L, He FL, Shi YK, Pan XH and Wang H: MicroRNA‑155 affects oxidative damage through regulating autophagy in endothelial cells. Oncol Lett 17: 2237-2243, 2019
APA
Chen, H., Liu Gao, M.Y., Zhang, L., He, F.L., Shi, Y.K., Pan, X.H., & Wang, H. (2019). MicroRNA‑155 affects oxidative damage through regulating autophagy in endothelial cells. Oncology Letters, 17, 2237-2243. https://doi.org/10.3892/ol.2018.9860
MLA
Chen, H., Liu Gao, M. Y., Zhang, L., He, F. L., Shi, Y. K., Pan, X. H., Wang, H."MicroRNA‑155 affects oxidative damage through regulating autophagy in endothelial cells". Oncology Letters 17.2 (2019): 2237-2243.
Chicago
Chen, H., Liu Gao, M. Y., Zhang, L., He, F. L., Shi, Y. K., Pan, X. H., Wang, H."MicroRNA‑155 affects oxidative damage through regulating autophagy in endothelial cells". Oncology Letters 17, no. 2 (2019): 2237-2243. https://doi.org/10.3892/ol.2018.9860