Open Access

MicroRNA‑26b acts as an antioncogene and prognostic factor in cervical cancer

  • Authors:
    • Lihong Wang
    • Wen Wang
    • Yuanyuan Wu
  • View Affiliations

  • Published online on: January 24, 2019     https://doi.org/10.3892/ol.2019.9965
  • Pages: 3418-3424
  • Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Cervical cancer is the second most frequent malignant neoplasm in women all over the world. Micro­RNA‑26b (miR‑26b) has been reported to be downregulated and play a great role in many malignancies, nevertheless, there are scarce studies on cervical cancer. The purpose of the present study was to detect how miR‑26b is involved in cervical carcinoma. Reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR) was utilized to detect the expression levels of miR‑26b and Jagged1 (JAG1) mRNA. Transwell assay was applied to calculate the cell migration and invasion capacity. Luciferase reporter assay was employed to determine JAG1 as a target of miR‑26b. The results revealed that miR‑26b is downregulated in cervical cancer tissues and cells compared with paracancerous tissues and normal cervical epithelial cells. The low expression of miR‑26b in cervical cancer demonstrated that miR‑26b inhibits cell migration and invasion, as measured by Transwell assay. JAG1 was verified to be a target of miR‑26b and have a negative correlation with miR‑26b, as detected by luciferase reporter assay. In addition, miR‑26b was found to suppress cell migration and invasion via mediating JAG1 expression, which impact is partially reversed by JAG1. In conclusion, miR‑26b suppresses cell migration and invasion of cervical cancer through directly targeting JAG1. It is suggested that miR‑26b/JAG1 axis may present a new target for the treatment of cervical cancer.
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March-2019
Volume 17 Issue 3

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Copy and paste a formatted citation
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Spandidos Publications style
Wang L, Wang W and Wu Y: MicroRNA‑26b acts as an antioncogene and prognostic factor in cervical cancer. Oncol Lett 17: 3418-3424, 2019
APA
Wang, L., Wang, W., & Wu, Y. (2019). MicroRNA‑26b acts as an antioncogene and prognostic factor in cervical cancer. Oncology Letters, 17, 3418-3424. https://doi.org/10.3892/ol.2019.9965
MLA
Wang, L., Wang, W., Wu, Y."MicroRNA‑26b acts as an antioncogene and prognostic factor in cervical cancer". Oncology Letters 17.3 (2019): 3418-3424.
Chicago
Wang, L., Wang, W., Wu, Y."MicroRNA‑26b acts as an antioncogene and prognostic factor in cervical cancer". Oncology Letters 17, no. 3 (2019): 3418-3424. https://doi.org/10.3892/ol.2019.9965