Open Access

Effect of asiatic acid on epithelial‑mesenchymal transition of human alveolar epithelium A549 cells induced by TGF‑β1

  • Authors:
    • Qingrong Cui
    • Juan Ren
    • Qingwei Zhou
    • Qinmei Yang
    • Bin Li
  • View Affiliations

  • Published online on: March 12, 2019     https://doi.org/10.3892/ol.2019.10140
  • Pages: 4285-4292
  • Copyright: © Cui et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Asiatic acid (AA) is a pentacyclic triterpenoid isolated from Centella asiatica (L.) Urban that possesses significant antitumor activities. In the present study, the mechanism of AA on transforming growth factor‑β1 (TGF‑β1)‑induced epithelial‑mesenchymal transition (EMT) was investigated in the lung cancer cell line A549. To do so, cell morphological alterations were observed and recorded at different time periods. Cells treated with TGF‑β1 were spindle‑shaped and characterized as stromal cells, whereas AA‑treated cells exhibited epithelial cell characteristics and increased intercellular adhesion. The MTT assay demonstrated that the high concentration of AA inhibited the viability of A549 cells treated with TGF‑β. In addition, the wound healing and Transwell assays revealed that AA inhibited TGF‑β1‑induced invasion and migration of A549 cells. Furthermore, AA treatment increased the mRNA and protein expression levels of E‑cadherin, and decreased the expression levels of snail family transcriptional repressor (Snail), N‑cadherin, vimentin and β‑catenin in TGF‑β1‑treated A549 cells. In conclusion, these results suggested that AA may inhibit TGF‑β1‑induced EMT in lung cancer through increased expression of E‑cadherin, and inhibition of Snail, N‑cadherin and vimentin expression.
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May-2019
Volume 17 Issue 5

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Cui Q, Ren J, Zhou Q, Yang Q and Li B: Effect of asiatic acid on epithelial‑mesenchymal transition of human alveolar epithelium A549 cells induced by TGF‑β1. Oncol Lett 17: 4285-4292, 2019
APA
Cui, Q., Ren, J., Zhou, Q., Yang, Q., & Li, B. (2019). Effect of asiatic acid on epithelial‑mesenchymal transition of human alveolar epithelium A549 cells induced by TGF‑β1. Oncology Letters, 17, 4285-4292. https://doi.org/10.3892/ol.2019.10140
MLA
Cui, Q., Ren, J., Zhou, Q., Yang, Q., Li, B."Effect of asiatic acid on epithelial‑mesenchymal transition of human alveolar epithelium A549 cells induced by TGF‑β1". Oncology Letters 17.5 (2019): 4285-4292.
Chicago
Cui, Q., Ren, J., Zhou, Q., Yang, Q., Li, B."Effect of asiatic acid on epithelial‑mesenchymal transition of human alveolar epithelium A549 cells induced by TGF‑β1". Oncology Letters 17, no. 5 (2019): 4285-4292. https://doi.org/10.3892/ol.2019.10140