CD24 induces the invasion of cholangiocarcinoma cells by upregulating CXCR4 and increasing the phosphorylation of ERK1/2

  • Authors:
    • Kawin Leelawat
    • Siriporn Keeratichamroen
    • Surang Leelawat
    • Rutaiwan Tohtong
  • View Affiliations

  • Published online on: September 17, 2013     https://doi.org/10.3892/ol.2013.1587
  • Pages: 1439-1446
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Cholangiocarcinoma is a malignant biliary tract tumor with an extremely poor prognosis. CD24 expression has been linked to the aggressiveness of cholangiocarcinoma cells and the adverse prognosis of cholangiocarcinoma patients. In the present study, the underlying mechanism of aggressive CD24+ cholangiocarcinoma cell behavior was elucidated. The magnetic‑activated cell sorting system was used to isolate CD24+ and CD24‑ cell populations from RMCCA1 cholangiocarcinoma cells. Using a human tumor metastasis PCR array, it was observed that numerous tumor‑associated genes were upregulated in the CD24+ cells, including CXC chemokine receptor type 4 (CXCR4). In addition, an intracellular signaling array demonstrated the activation of extracellular signal‑regulated kinase (ERK)1/2, which is downstream of the CXCR4 signaling cascade, in the CD24+ cells. Inhibition of CXCR4 or ERK1/2 significantly inhibited the motility and invasiveness of the CD24+ cells. The present study indicates that CXCR4 and ERK1/2 are induced by CD24 and that these proteins are associated with cholangiocarcinoma cell invasion.
View Figures
View References

Related Articles

Journal Cover

November 2013
Volume 6 Issue 5

Print ISSN: 1792-1074
Online ISSN:1792-1082

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Leelawat K, Keeratichamroen S, Leelawat S and Tohtong R: CD24 induces the invasion of cholangiocarcinoma cells by upregulating CXCR4 and increasing the phosphorylation of ERK1/2. Oncol Lett 6: 1439-1446, 2013
APA
Leelawat, K., Keeratichamroen, S., Leelawat, S., & Tohtong, R. (2013). CD24 induces the invasion of cholangiocarcinoma cells by upregulating CXCR4 and increasing the phosphorylation of ERK1/2. Oncology Letters, 6, 1439-1446. https://doi.org/10.3892/ol.2013.1587
MLA
Leelawat, K., Keeratichamroen, S., Leelawat, S., Tohtong, R."CD24 induces the invasion of cholangiocarcinoma cells by upregulating CXCR4 and increasing the phosphorylation of ERK1/2". Oncology Letters 6.5 (2013): 1439-1446.
Chicago
Leelawat, K., Keeratichamroen, S., Leelawat, S., Tohtong, R."CD24 induces the invasion of cholangiocarcinoma cells by upregulating CXCR4 and increasing the phosphorylation of ERK1/2". Oncology Letters 6, no. 5 (2013): 1439-1446. https://doi.org/10.3892/ol.2013.1587