JSI124 inhibits breast cancer cell growth by suppressing the function of B cells via the downregulation of signal transducer and activator of transcription 3

  • Authors:
    • Yi Ren
    • Kun Yu
    • Su'an Sun
    • Zhi Li
    • Jin Yuan
    • Xue Dong Han
    • Jianhua Shi
    • Linlin Zhen
  • View Affiliations

  • Published online on: June 4, 2014     https://doi.org/10.3892/ol.2014.2221
  • Pages: 928-932
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Abstract

JSI‑124, also known as cucurbitacin I, is a selective inhibitor of Janus kinase/signal transducer and activator of transcription 3 (JAK/STAT3), and in vitro and in vivo studies have found that it has anti‑tumor and anti‑proliferative properties. However, the role of JSI124 in tumor‑associated B cells has yet to be elucidated. The present study demonstrated that STAT3 is significantly activated in the B cells of patients with breast cancer. Furthermore, a 4T1 tumor‑bearing mouse model revealed that JSI124 effectively inhibited tumor growth. Moreover, the STAT3 levels in the B cells of the JSI124‑treated mice were found to be significantly decreased. B cells from normal Balb/c mice, the 4T1‑bearing mice and the JSI124‑treated 4T1 mice were purified and intravenously injected into the 4T1‑bearing Balb/c mice. Tumor growth data showed that the 4T1 tumor mouse‑derived B cells, which exhibited a higher level of STAT3, promoted tumor growth, while the JSI124‑treated 4T1 mouse‑derived B cells had a tumor suppressor function. Furthermore, the B cells from the normal Balb/c mice were treated with phosphate‑buffered saline, JSI124 and 4T1 tumor cells, then the B cell STAT3 levels were analyzed. Following injection into the 4T1 mice, the 4T1 cell‑treated B cells were observed to enhance tumor growth, while the JSI124‑treated B cells were found to inhibit the growth of 4T1 tumors in vivo. These findings show a novel role of JSI124 in tumor suppression through the downregulation of the expression of STAT3 in tumor‑associated B cells.
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August-2014
Volume 8 Issue 2

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Ren Y, Yu K, Sun S, Li Z, Yuan J, Han XD, Shi J and Zhen L: JSI124 inhibits breast cancer cell growth by suppressing the function of B cells via the downregulation of signal transducer and activator of transcription 3. Oncol Lett 8: 928-932, 2014
APA
Ren, Y., Yu, K., Sun, S., Li, Z., Yuan, J., Han, X.D. ... Zhen, L. (2014). JSI124 inhibits breast cancer cell growth by suppressing the function of B cells via the downregulation of signal transducer and activator of transcription 3. Oncology Letters, 8, 928-932. https://doi.org/10.3892/ol.2014.2221
MLA
Ren, Y., Yu, K., Sun, S., Li, Z., Yuan, J., Han, X. D., Shi, J., Zhen, L."JSI124 inhibits breast cancer cell growth by suppressing the function of B cells via the downregulation of signal transducer and activator of transcription 3". Oncology Letters 8.2 (2014): 928-932.
Chicago
Ren, Y., Yu, K., Sun, S., Li, Z., Yuan, J., Han, X. D., Shi, J., Zhen, L."JSI124 inhibits breast cancer cell growth by suppressing the function of B cells via the downregulation of signal transducer and activator of transcription 3". Oncology Letters 8, no. 2 (2014): 928-932. https://doi.org/10.3892/ol.2014.2221