Trichostatin A with adenovirus-mediated p53 gene transfer synergistically induces apoptosis in breast cancer cell line MDA-MB-231

  • Authors:
    • Sei-Ichiro Nakajima
    • Hiroto Niizeki
    • Mitsuo Tada
    • Koji Nakagawa
    • Satoshi Kondo
    • Futoshi Okada
    • Masanobu Kobayashi
  • View Affiliations

  • Published online on: July 1, 2009     https://doi.org/10.3892/or_00000417
  • Pages: 143-148
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Abstract

Although the p53 anti-oncogene is an important target for gene therapy of cancer, some cancers are resistant to p53 gene transfer. For this reason, it is important to find effective drugs to enhance cytotoxic effects of p53 gene transfer. Recent reports demonstrated that some histone deacetylase inhibitors in combination with p53 gene therapy induced apoptosis in certain cancer cells more efficiently than p53 gene therapy alone. We investigated whether histone deacetylase inhibitor Trichostatin A (TSA), in combination with p53 gene transfer could synergistically induce apoptosis in the breast cancer cell line MDA-MB-231. Whereas the adenovirus-expressing p53 (Ad-p53) by itself at up to 100 multiplicity of infection (MOI) induced apoptosis at a low level, Ad-p53 in combination with TSA synergistically induced apoptosis at a higher level in MDA-MB-231 cells than TSA or Ad-p53 alone. However, the combination of Ad-p53 and TSA did not enhance the expressions of p53 or p53-induced genes that are involved in apoptosis, and synergistically reduced the mitochondrial membrane potential and enhanced caspase-3 activity. These results suggest that TSA have synergistic effects on the induction of apoptosis in MDA-MB-231 cells when combined with p53 gene transfer.

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July 2009
Volume 22 Issue 1

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Nakajima S, Niizeki H, Tada M, Nakagawa K, Kondo S, Okada F and Kobayashi M: Trichostatin A with adenovirus-mediated p53 gene transfer synergistically induces apoptosis in breast cancer cell line MDA-MB-231. Oncol Rep 22: 143-148, 2009
APA
Nakajima, S., Niizeki, H., Tada, M., Nakagawa, K., Kondo, S., Okada, F., & Kobayashi, M. (2009). Trichostatin A with adenovirus-mediated p53 gene transfer synergistically induces apoptosis in breast cancer cell line MDA-MB-231. Oncology Reports, 22, 143-148. https://doi.org/10.3892/or_00000417
MLA
Nakajima, S., Niizeki, H., Tada, M., Nakagawa, K., Kondo, S., Okada, F., Kobayashi, M."Trichostatin A with adenovirus-mediated p53 gene transfer synergistically induces apoptosis in breast cancer cell line MDA-MB-231". Oncology Reports 22.1 (2009): 143-148.
Chicago
Nakajima, S., Niizeki, H., Tada, M., Nakagawa, K., Kondo, S., Okada, F., Kobayashi, M."Trichostatin A with adenovirus-mediated p53 gene transfer synergistically induces apoptosis in breast cancer cell line MDA-MB-231". Oncology Reports 22, no. 1 (2009): 143-148. https://doi.org/10.3892/or_00000417