Altered cytokine levels and increased CD4+CD57+ T cells in the peripheral blood of hepatitis C virus-related hepatocellular carcinoma patients

  • Authors:
    • Tatsuya Shiraki
    • Eiji Takayama
    • Hirohito Magari
    • Takahiro Nakata
    • Takao Maekita
    • Shotaro Enomoto
    • Yoshiyuki Mori
    • Naoki Shingaki
    • Kosaku Moribata
    • Hisanobu Deguchi
    • Kazuki Ueda
    • Izumi Inoue
    • Masako Mizuno-Kamiya
    • Koji Yashiro
    • Mikitaka Iguchi
    • Hideyuki Tamai
    • Yasunaga Kameyama
    • Jun Kato
    • Nobuo Kondoh
    • Masao Ichinose
  • View Affiliations

  • Published online on: April 12, 2011     https://doi.org/10.3892/or.2011.1258
  • Pages: 201-208
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Abstract

Although CD57+ lymphocytes are closely correlated with prognosis in various cancers, the role of subsets of CD57+ cells in hepatitis C virus (HCV)-related hepatocellular carcinoma (HCC) is unclear. In the present study, peripheral blood (PB) from HCV-related HCC patients was analyzed. Plasma cytokine levels and in vitro cytokine-producing capabilities were analyzed with enzyme-linked immunosorbent assays, and CD57+ cell subsets were studied using a multi-color FACS system. Interferon (IFN)-γ was undetectable in the plasma of patients with tumors at any stage, whereas the plasma levels of tumor necrosis factor (TNF)-α, interleukin (IL)-10 and IL-18, but not that of IL-12, were significantly higher in stage IV patients compared to patients with earlier-stage tumors. In contrast, the IFN-γ-producing capability of PB was highest in stage I patients and gradually decreased with tumor progression. The IL-10-, IL-18- and IL-12-producing capabilities of PB increased from stage I to III. However, PB-TNF-α, IL-10- and IL-18-producing capabilities were reduced in stage IV patients, probably due to repeated anti-cancer treatments. The percentage of CD4+CD57+αβTCR+ cells (CD4+CD57+ T cells) in peripheral blood lymphocytes (PBLs) increased with tumor progression. Moreover, the percentage of CD4+CD57+ T cells in PBLs and the ratio of CD4+CD57+ T cells to CD4+αβTCR+ cells (CD4+ T cells), but not that of CD4+CD57+ T cells to CD57+αβTCR+ cells (CD57+ T cells), showed a significant inverse correlation with PB-IFN-γ-producing capability. The present results suggest that an increase in CD4+CD57+ T cells controls the capability of PB to produce the anti-tumor cytokine IFN-γ and that PB-IFN-γ production is impaired with HCC tumor progression.

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July 2011
Volume 26 Issue 1

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Spandidos Publications style
Shiraki T, Takayama E, Magari H, Nakata T, Maekita T, Enomoto S, Mori Y, Shingaki N, Moribata K, Deguchi H, Deguchi H, et al: Altered cytokine levels and increased CD4+CD57+ T cells in the peripheral blood of hepatitis C virus-related hepatocellular carcinoma patients. Oncol Rep 26: 201-208, 2011
APA
Shiraki, T., Takayama, E., Magari, H., Nakata, T., Maekita, T., Enomoto, S. ... Ichinose, M. (2011). Altered cytokine levels and increased CD4+CD57+ T cells in the peripheral blood of hepatitis C virus-related hepatocellular carcinoma patients. Oncology Reports, 26, 201-208. https://doi.org/10.3892/or.2011.1258
MLA
Shiraki, T., Takayama, E., Magari, H., Nakata, T., Maekita, T., Enomoto, S., Mori, Y., Shingaki, N., Moribata, K., Deguchi, H., Ueda, K., Inoue, I., Mizuno-Kamiya, M., Yashiro, K., Iguchi, M., Tamai, H., Kameyama, Y., Kato, J., Kondoh, N., Ichinose, M."Altered cytokine levels and increased CD4+CD57+ T cells in the peripheral blood of hepatitis C virus-related hepatocellular carcinoma patients". Oncology Reports 26.1 (2011): 201-208.
Chicago
Shiraki, T., Takayama, E., Magari, H., Nakata, T., Maekita, T., Enomoto, S., Mori, Y., Shingaki, N., Moribata, K., Deguchi, H., Ueda, K., Inoue, I., Mizuno-Kamiya, M., Yashiro, K., Iguchi, M., Tamai, H., Kameyama, Y., Kato, J., Kondoh, N., Ichinose, M."Altered cytokine levels and increased CD4+CD57+ T cells in the peripheral blood of hepatitis C virus-related hepatocellular carcinoma patients". Oncology Reports 26, no. 1 (2011): 201-208. https://doi.org/10.3892/or.2011.1258