Chemotherapy-induced modification of microRNA expression in esophageal cancer

  • Authors:
    • Richard Hummel
    • Tingting Wang
    • David I. Watson
    • Michael Z. Michael
    • Mark Van der Hoek
    • Joerg Haier
    • Damian J. Hussey
  • View Affiliations

  • Published online on: July 4, 2011     https://doi.org/10.3892/or.2011.1381
  • Pages: 1011-1017
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Abstract

Neoadjuvant chemotherapy is often used in the treatment of advanced esophageal cancer. In this study, we determined the impact of chemotherapy on microRNA (miRNA) expression in esophageal cancer cells, and whether identified changes might have biological relevance. Two esophageal carcinoma cell lines (one adenocarcinoma and one squamous cell carcinoma) were treated with cisplatin or 5-fluorouracil for 24 or 72 h. RNA was extracted from cells following 24-h treatment, and used for microarray studies. Promising miRNA candidates were selected for RT-PCR validation. Target prediction using TargetScan, combined with bioinformatic analysis (Ingenuity Pathway Analysis, IPA), was performed to evaluate the implications of the altered miRNA expression. Thirteen miRNAs (miR-199a-5p, miR-302f, miR-320a, miR-342-3p, miR-425, miR-455-3p, miR-486-3p, miR-519c-5p, miR-548d-5p, miR-617, miR-758, miR-766, miR-1286) were deregulated after 24- and/or 72-h treatment in both cell lines, and most miRNAs presented similar expression changes after short- or long-term exposure. IPA revealed that the major networks which incorporate the predicted targets, include functions such as ‘Cell death’, ‘Cell cycle’, ‘Cellular growth and proliferation’, ‘DNA replication, recombination, and repair’ and ‘Drug metabolism’. Cisplatin or 5-fluorouracil alter miRNA expression in esophageal cancer cells. IPA suggests that these miRNAs may target molecular pathways involved in cell survival after chemotherapy.

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October 2011
Volume 26 Issue 4

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Copy and paste a formatted citation
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Spandidos Publications style
Hummel R, Wang T, Watson DI, Michael MZ, Van der Hoek M, Haier J and Hussey DJ: Chemotherapy-induced modification of microRNA expression in esophageal cancer. Oncol Rep 26: 1011-1017, 2011
APA
Hummel, R., Wang, T., Watson, D.I., Michael, M.Z., Van der Hoek, M., Haier, J., & Hussey, D.J. (2011). Chemotherapy-induced modification of microRNA expression in esophageal cancer. Oncology Reports, 26, 1011-1017. https://doi.org/10.3892/or.2011.1381
MLA
Hummel, R., Wang, T., Watson, D. I., Michael, M. Z., Van der Hoek, M., Haier, J., Hussey, D. J."Chemotherapy-induced modification of microRNA expression in esophageal cancer". Oncology Reports 26.4 (2011): 1011-1017.
Chicago
Hummel, R., Wang, T., Watson, D. I., Michael, M. Z., Van der Hoek, M., Haier, J., Hussey, D. J."Chemotherapy-induced modification of microRNA expression in esophageal cancer". Oncology Reports 26, no. 4 (2011): 1011-1017. https://doi.org/10.3892/or.2011.1381