Sleeping Beauty transposon system harboring HRAS, c-Myc and shp53 induces sarcomatoid carcinomas in mouse skin

The Sleeping Beauty transposon system is used as a tool for insertional mutagenesis and oncogenesis. However, little is known about the exact histological phenotype of the tumors induced. Thus, we used immunohistochemical markers to enable histological identification of the type of tumor induced by subcutaneous injection of the HRAS, c-Myc and shp53 oncogenes in female C57BL/6 mice. The tumor was removed when it reached 100 mm3 in volume. Subsequently, we used 13 immunohistochemical markers to histologically identify the tumor type. The results suggested that the morphology of the tumor was similar to that of sarcomatoid carcinoma.


Introduction
The Sleeping Beauty (SB) system is a genetically engineered insertional mutagenesis system that consists of two components: a transposon, which is a series of DNA mobile elements flanked by indirect repeat sequences, and SB transposase, which catalyzes the mobilization and reintegration of transposon into mouse genomic DNA (1,2). Integration into the host chromosome provides prolonged expression of the transgene. In addition, the SB system has the advantages of both viral and non-viral vector systems (3). For this reason, the SB system has attracted much attention as a promising delivery system, as well as a discovery tool for cancer-associated genes. Its ability to achieve long-term in vivo expression suggests that the somatic integration of oncogenes using SB is a feasible approach to the development of molecularly defined tumorigenic mouse models (4). Although xenograft models and genetically engineered mice are able to mimic human cancer progression (5), the present mouse model systems do not correspond to humans with regard to genetic backgrounds (i.e., different genetic variants, genetic mutations and subsequent protein expression) (6)(7)(8). A hallmark of human cancer is genetic complexity, meaning a number of different mutations are commonly involved (9). The complex genetic alterations in different types of cancer cause various histological subtypes and can explain the heterogeneous nature of a given neoplasm (9). Carlson et al (4) succeeded in eliciting tumor formation in mouse hepatocytes by hydrodynamic injection of a transposon containing an activated NRAS oncogene. Although those investigators studied the simple feature of the tumors induced by insertional mutagenesis, the exact origins and tumor subtypes have yet to be determined.
In the present study, the c-Myc, HRAS and shp53 oncogenes were delivered with SB into C57BL/6 mice. A malignant undifferentiated tumor was subsequently identified at the injection site on the subcutis of the right side of the lower ventral abdominal area. The nature of the tumor was identified by histological and immunohistochemical methods (Table I).

DNA plasmid injections.
Animals received a mixture of three types of transposon and the plasmid encoding the transposase, as detailed above. The molar ratio of transposase-encoding plasmids to transposon plasmids was 1:2. First, three types of transposon were mixed in equal amounts in total 50 µg and then the transposase encoding plasmids was added to the transposon mixture with 50 µl of phosphate-buffered saline (PBS). The DNA mixture was collected with an insulin syringe (31 G) and injected subcutaneously near the right inferior mammary gland. Transposon genes and transposase were regarded to be 7,000 and 5,000 kb in size, respectively, rounded to the nearest kb.
Animal PET imaging Radiopharmaceutical preparation. Decay-corrected radiochemical yields ranged from 60 to 70% and after highperformance liquid chromatography (HPLC) purification, the radiochemical purity was 98±1.2% (mean ± SD Tumor monitoring and necropsy. Mice were carefully examined three times each week to detect tumors. Tumors were measured using a digital caliper. The tumor volume (v) in mm 3 was calculated using the formula v = L x W 2 /2, where L is the longest diameter and W is the tumor length that is perpendicular to L. When the tumor reached 100 mm 3 in volume, the mice were humanely euthanized and subjected to a necropsy in which the tumor was excised with the circumferential tissue for histopathological examination.
Detection of the expression of genes. Expression of the c-Myc and HRAS genes was identified by immunohistochemistry with anti-c-Myc (1:1,000, Abcam) and HRAS (1:1,000, Abcam) antibodies, respectively. For the detection of the shp53 gene, an anti-GFP antibody (1:4,000, Abcam) was used as a GFP gene was contained in the shp53 DNA plasmid as a reporter gene.

Results
Tumor observation. Recipients (12/12) of the three transposons and the SB transposase developed single or coupled, nodular, subcutaneous neoplasms ~30 days after injection (Fig. 2). Each tumor grew rapidly from initial detection to eventually reach a large volume.
Animal PET imaging. Different dimensions, both transverse and longitudinal, of PET-CT images for subcutaneous neoplasms were detected on the injection sites. The tumors are observed as a red-pinkish-colored small ball (Fig. 3).
Gross and microscopic findings. A well-demarcated, ovoid nodule was located in the subcutaneous soft tissue where the   DNA plasmids were injected. Metastatic foci in non-injected sites were not observed. The tumor showed high cellularity with abundant mitoses and apoptosis. Both epithelial and mesenchymal components were evident. The tumor cells were undifferentiated with pleomorphic features characterized by round to oval cells with pale basophilic cytoplasm and hyperchromatic nuclei with prominent nucleoli (Fig. 4). Multinucleated giant cells were occasionally identified. Areas of necrosis were also evident in the central part of the tumor. However, the overlying skin and its associated adnexa, including hair follicles, sebaceous glands and mammary glands, did not exhibit dysplastic changes. All the tumors had the same morphological features.
Immunohistochemical findings. As summarized in Table II, only pan-cytokeratin was found to be positively expressed by the tumor cells (Fig. 5), suggesting the epithelial origin of the tumor. The other markers were completely negative, except in the tumor-associated stroma. Consequently, we diagnosed this tumor as a sarcomatoid carcinoma.

Identification of gene expression in the tumor.
Expression of the c-Myc and HRAS oncogenes was demonstrated by immunohistochemical positivity for the c-Myc and HRAS antibodies, respectively. A positive signal was detected with an anti-GFP antibody, indicating expression of the shp53 DNA.

Discussion
In the present study, we induced tumors in mice via the injection of transposons encoding three oncogenes and a plasmid-expressing transposase. The histological features of the induced tumor were poorly differentiated, thus we used immunohistochemical markers to characterize this lesion. Multinucleated giant cells were occasionally identified. Areas of necrosis were also identified in the central part of the tumor. Notably, the tumor expressed only pan-cytokeratin and all other markers used in this study were completely negative. As a result, we diagnosed the tumor as a sarcomatoid carcinoma.
To the best of our knowledge, there are no spontaneously occurring subcutis malignant epithelial tumors that morphologically resemble the tumor induced in this mouse study. The origin of this tumor remains unknown as the overlying epidermis and associated adnexa were not histologically dysplastic. Mammary tissues were also intact and normal mammary tissues were embedded within the tumor. This finding is consistent with the fact that mammary tissues lack response to the SB transposon system. Although this system has recently been shown to be able to induce various types of SB transposon-induced tumors, certain types of tumors such as those of the lung, mammary gland, prostate and pancreas, have not yet been generated with the SB transposon system in the mouse (10).
As the oncogenes were injected into the subcutis area, the origin of the tumor is assumed to be the skin adnexa, although this could not be precisely defined. c-Myc overexpression is associated with an undifferentiated phenotype in cultured astrocytes (11) and gastric carcinomas (12). In addition, c-Myc is downregulated during myogenic differentiation (13,14). HRAS overexpression is also associated with an anaplastic phenotype in mammary adenocarcinomas (15). Therefore, we hypothesize that injection of these oncogenes induced the growth of tumors with an undifferentiated phenotype.
The tumors induced in this study appeared to be of mesenchymal origin morphologically. Features of the tumors including undifferentiated pleomorphic cells characterized by hyperchromatic nuclei with prominent nucleoli and basophilic spindle-shaped cytoplasm resembled sarcomatous tumor. However, results of immunohistochemical staining showed this tumor to be carcinoma. p53 gene knockout is involved in presenting sarcomatoid features, as recent studies showed that tumor-suppressor gene mutations within the stroma allowed mesenchymal proliferation (16).
Insertional mutagenesis is commonly used in gene discovery studies in the field of oncology. This method is easy and more rapid compared to conventional genetic engineering (e.g., gene knockout). As a result, previous studies have focused on the use of the SB transposon system for insertional mutagenesis (17,18). However, detailed histological charac terization of the induced tumors has not previously been performed. Thus, this study is, to the best of our knowledge, the first report of the histological and immunohistochemical identification of subcutaneous tumors induced by transposable elements.