Allyl isothiocyanate inhibits cell metastasis through suppression of the MAPK pathways in epidermal growth factor‑stimulated HT29 human colorectal adenocarcinoma cells

  • Authors:
    • Kuang-Chi Lai
    • Chi-Cheng Lu
    • Yih-Jing Tang
    • Jo-Hua Chiang
    • Daih-Huang Kuo
    • Fu-An Chen
    • I-Li Chen
    • Jai-Sing Yang
  • View Affiliations

  • Published online on: November 20, 2013     https://doi.org/10.3892/or.2013.2865
  • Pages: 189-196
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Abstract

Allyl isothiocyanate (AITC) has been found to present sources from consumed cruciferous vegetables. AITC is known to possess pharmacological and anticancer activities. The present study was designed to test the hypothesis that AITC suppressed the invasion and migration of epidermal growth factor (EGF)-stimulated HT29 cells and to elucidate the mechanisms for the antimetastatic abilities in vitro. The invasion and migration of EGF-stimulated HT29 cells were determined individually by Transwell cell invasion and wound-healing assays. Our results showed that AITC effectively inhibited both the invasive and migratory ability of HT29 cells. Furthermore, AITC downregulated the protein levels of matrix metalloproteinase-2 (MMP-2), MMP-9 and mitogen-activated protein kinases (MAPKs) (p-JNK, p-ERK and p-p38) by western blot analysis in HT29 cells following EGF induction. Thus, the metastatic responses in AITC-treated HT29 cells after EGF stimulation were mediated by the MMP-2/-9 and MAPK signaling pathways. We also used gene expression microarrays to investigate the gene levels related to cell growth, G-protein coupled receptor, angiogenesis, cell adhesion, cell cycle and mitosis, cell migration, cytoskeleton organization, DNA damage and repair, transcription and translation, EGFR and PKB/mTOR signals. In summary, it is possible that AITC suppresses the invasion and migration of EGF-induced HT29 cells, resulting from MMP-2/-9 and MAPKs. Hence, AITC may be beneficial in the treatment of human colorectal adenocarcinoma in the future.
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2014-January
Volume 31 Issue 1

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Lai K, Lu C, Tang Y, Chiang J, Kuo D, Chen F, Chen I and Yang J: Allyl isothiocyanate inhibits cell metastasis through suppression of the MAPK pathways in epidermal growth factor‑stimulated HT29 human colorectal adenocarcinoma cells. Oncol Rep 31: 189-196, 2014
APA
Lai, K., Lu, C., Tang, Y., Chiang, J., Kuo, D., Chen, F. ... Yang, J. (2014). Allyl isothiocyanate inhibits cell metastasis through suppression of the MAPK pathways in epidermal growth factor‑stimulated HT29 human colorectal adenocarcinoma cells. Oncology Reports, 31, 189-196. https://doi.org/10.3892/or.2013.2865
MLA
Lai, K., Lu, C., Tang, Y., Chiang, J., Kuo, D., Chen, F., Chen, I., Yang, J."Allyl isothiocyanate inhibits cell metastasis through suppression of the MAPK pathways in epidermal growth factor‑stimulated HT29 human colorectal adenocarcinoma cells". Oncology Reports 31.1 (2014): 189-196.
Chicago
Lai, K., Lu, C., Tang, Y., Chiang, J., Kuo, D., Chen, F., Chen, I., Yang, J."Allyl isothiocyanate inhibits cell metastasis through suppression of the MAPK pathways in epidermal growth factor‑stimulated HT29 human colorectal adenocarcinoma cells". Oncology Reports 31, no. 1 (2014): 189-196. https://doi.org/10.3892/or.2013.2865