Open Access

A novel gene expression system strongly enhances the anticancer effects of a REIC/Dkk-3-encoding adenoviral vector

  • Authors:
    • Masami Watanabe
    • Masakiyo Sakaguchi
    • Rie Kinoshita
    • Haruki Kaku
    • Yuichi Ariyoshi
    • Hideo Ueki
    • Ryuta Tanimoto
    • Shin Ebara
    • Kazuhiko Ochiai
    • Junichiro Futami
    • Shun-Ai Li
    • Peng Huang
    • Yasutomo Nasu
    • Nam-Ho Huh
    • Hiromi Kumon
  • View Affiliations

  • Published online on: December 31, 2013     https://doi.org/10.3892/or.2013.2958
  • Pages: 1089-1095
  • Copyright: © Watanabe et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].

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Abstract

Gene expression systems with various promoters, including the cytomegalovirus (CMV) promoter, have been developed to increase the gene expression in a variety of normal and cancer cells. In particular, in the clinical trials of cancer gene therapy, a more efficient and robust gene expression system is required to achieve sufficient therapeutic outcomes. By inserting the triple translational enhancer sequences of human telomerase reverse transcriptase (hTERT), Simian virus 40 (SV40) and CMV downstream of the sequence of the BGH polyA, we were able to develop a novel gene expression system that significantly enhances the expression of the genes of interest. We termed this novel gene expression cassette the super gene expression (SGE) system, and herein verify the utility of the SGE cassette for a replication-deficient adenoviral vector. We newly developed an adenoviral vector expressing the tumor suppressor, reduced expression in immortalized cells (REIC)/Dickkopf-3 (Dkk-3), based on the CMV promoter-driven SGE system (Ad-SGE-REIC) and compared the therapeutic utility of Ad-SGE-REIC with that of the conventional adenoviral vectors (Ad-CMV-REIC or Ad-CAG-REIC). The results demonstrated that the CMV promoter-SGE system allows for more potent gene expression, and that the Ad-SGE-REIC is superior to conventional adenoviral systems in terms of the REIC protein expression and therapeutic effects. Since the SGE cassette can be applied for the expression of various therapeutic genes using various vector systems, we believe that this novel system will become an innovative tool in the field of gene expression and gene therapy.
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2014-March
Volume 31 Issue 3

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Watanabe M, Sakaguchi M, Kinoshita R, Kaku H, Ariyoshi Y, Ueki H, Tanimoto R, Ebara S, Ochiai K, Futami J, Futami J, et al: A novel gene expression system strongly enhances the anticancer effects of a REIC/Dkk-3-encoding adenoviral vector. Oncol Rep 31: 1089-1095, 2014
APA
Watanabe, M., Sakaguchi, M., Kinoshita, R., Kaku, H., Ariyoshi, Y., Ueki, H. ... Kumon, H. (2014). A novel gene expression system strongly enhances the anticancer effects of a REIC/Dkk-3-encoding adenoviral vector. Oncology Reports, 31, 1089-1095. https://doi.org/10.3892/or.2013.2958
MLA
Watanabe, M., Sakaguchi, M., Kinoshita, R., Kaku, H., Ariyoshi, Y., Ueki, H., Tanimoto, R., Ebara, S., Ochiai, K., Futami, J., Li, S., Huang, P., Nasu, Y., Huh, N., Kumon, H."A novel gene expression system strongly enhances the anticancer effects of a REIC/Dkk-3-encoding adenoviral vector". Oncology Reports 31.3 (2014): 1089-1095.
Chicago
Watanabe, M., Sakaguchi, M., Kinoshita, R., Kaku, H., Ariyoshi, Y., Ueki, H., Tanimoto, R., Ebara, S., Ochiai, K., Futami, J., Li, S., Huang, P., Nasu, Y., Huh, N., Kumon, H."A novel gene expression system strongly enhances the anticancer effects of a REIC/Dkk-3-encoding adenoviral vector". Oncology Reports 31, no. 3 (2014): 1089-1095. https://doi.org/10.3892/or.2013.2958