Ligustrum lucidum Ait. fruit extract induces apoptosis and cell senescence in human hepatocellular carcinoma cells through upregulation of p21

  • Authors:
    • Bing Hu
    • Qin Du
    • Shan Deng
    • Hong-Mei  An
    • Chuan-Fang  Pan
    • Ke‑Ping  Shen
    • Ling  Xu
    • Meng-Meng Wei
    • Shuang-Shuang Wang
  • View Affiliations

  • Published online on: July 7, 2014     https://doi.org/10.3892/or.2014.3312
  • Pages: 1037-1042
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Abstract

Nü-zhen-zi, the fruit of Ligustrum lucidum Ait., is one of the most frequently used liver Yin tonifying Chinese herbs for the treatment of liver cancer. However, the effect of Ligustrum lucidum fruit on hepatocarcinoma cells remains unknown. In the present study, we evaluated the effects of a Ligustrum lucidum fruit extract (LLFE) on human hepatocellular carcinoma Bel-7402 cells. The results showed that LLFE inhibited the proliferation of the Bel-7402 cells in a dose- and time-dependent manner. LLFE induced apoptosis in Bel-7402 cells accompanied by activation of caspase-3, -8 and -9. LLFE-induced apoptosis was completely abrogated by a pan caspase inhibitor, Z-VAD-FMK. LLFE treatment also caused a large and flat morphologic cellular change, positive SA-β-gal staining, and G0/G1 phase cell cycle arrest in the Bel-7402 cells, accompanied by upregulation of p21 and downregulation of RB phosphorylation. Specific knockdown of p21 expression by RNA interference partially abrogated LLFE-induced apoptosis, and significantly abrogated LLFE-induced cell senescence. These observations suggest that Nü-zhen-zi is a potential anticancer herb and support the traditional use of Nü-zhen-zi for hepatocarcinoma treatment.
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September-2014
Volume 32 Issue 3

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Hu B, Du Q, Deng S, An H, Pan C, Shen KP, Xu L, Wei M and Wang S: Ligustrum lucidum Ait. fruit extract induces apoptosis and cell senescence in human hepatocellular carcinoma cells through upregulation of p21. Oncol Rep 32: 1037-1042, 2014
APA
Hu, B., Du, Q., Deng, S., An, H., Pan, C., Shen, K. ... Wang, S. (2014). Ligustrum lucidum Ait. fruit extract induces apoptosis and cell senescence in human hepatocellular carcinoma cells through upregulation of p21. Oncology Reports, 32, 1037-1042. https://doi.org/10.3892/or.2014.3312
MLA
Hu, B., Du, Q., Deng, S., An, H., Pan, C., Shen, K., Xu, L., Wei, M., Wang, S."Ligustrum lucidum Ait. fruit extract induces apoptosis and cell senescence in human hepatocellular carcinoma cells through upregulation of p21". Oncology Reports 32.3 (2014): 1037-1042.
Chicago
Hu, B., Du, Q., Deng, S., An, H., Pan, C., Shen, K., Xu, L., Wei, M., Wang, S."Ligustrum lucidum Ait. fruit extract induces apoptosis and cell senescence in human hepatocellular carcinoma cells through upregulation of p21". Oncology Reports 32, no. 3 (2014): 1037-1042. https://doi.org/10.3892/or.2014.3312