Diallyl disulfide induces G2/M arrest and promotes apoptosis through the p53/p21 and MEK-ERK pathways in human esophageal squamous cell carcinoma

  • Authors:
    • Xiaoran Yin
    • Rong Zhang
    • Cheng Feng
    • Jun Zhang
    • Dong Liu
    • Kun Xu
    • Xijing Wang
    • Shuqun Zhang
    • Zongfang Li
    • Xinlian Liu
    • Hongbing Ma
  • View Affiliations

  • Published online on: July 25, 2014     https://doi.org/10.3892/or.2014.3361
  • Pages: 1748-1756
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Abstract

Esophageal squamous cell carcinoma (ESCC) is an aggressive tumor with high incidence and mortality worldwide. Diallyl disulfide (DADS) is a natural organosulfur compound, isolated from garlic. In this study, MTT assay showed that DADS significantly reduced cell viability in a dose- and time-dependent manner in ESCC cells, with lower toxicity in normal liver cells. Cell cycle analysis revealed that DADS made G2/M phase arrest. Molecular analysis suggested that this cell cycle arrest was likely made by the decrease of cyclin B1, cdc2, p-cdc2, cdc25c in concomitance with activation of the p53/p21 pathway. Apoptosis was detected by Annexin V/PI staining. The molecule markers showed that DADS induced apoptosis through activating caspases, altering the Bax/Bcl-2 balance and suppressing the MEK-ERK pathway. Our data indicated that DADS has the potential to be an effective and safe anticancer agent for ESCC therapy in the near future.
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October 2014
Volume 32 Issue 4

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Yin X, Zhang R, Feng C, Zhang J, Liu D, Xu K, Wang X, Zhang S, Li Z, Liu X, Liu X, et al: Diallyl disulfide induces G2/M arrest and promotes apoptosis through the p53/p21 and MEK-ERK pathways in human esophageal squamous cell carcinoma. Oncol Rep 32: 1748-1756, 2014
APA
Yin, X., Zhang, R., Feng, C., Zhang, J., Liu, D., Xu, K. ... Ma, H. (2014). Diallyl disulfide induces G2/M arrest and promotes apoptosis through the p53/p21 and MEK-ERK pathways in human esophageal squamous cell carcinoma. Oncology Reports, 32, 1748-1756. https://doi.org/10.3892/or.2014.3361
MLA
Yin, X., Zhang, R., Feng, C., Zhang, J., Liu, D., Xu, K., Wang, X., Zhang, S., Li, Z., Liu, X., Ma, H."Diallyl disulfide induces G2/M arrest and promotes apoptosis through the p53/p21 and MEK-ERK pathways in human esophageal squamous cell carcinoma". Oncology Reports 32.4 (2014): 1748-1756.
Chicago
Yin, X., Zhang, R., Feng, C., Zhang, J., Liu, D., Xu, K., Wang, X., Zhang, S., Li, Z., Liu, X., Ma, H."Diallyl disulfide induces G2/M arrest and promotes apoptosis through the p53/p21 and MEK-ERK pathways in human esophageal squamous cell carcinoma". Oncology Reports 32, no. 4 (2014): 1748-1756. https://doi.org/10.3892/or.2014.3361