Bone marrow-derived mesenchymal stem cells co‑expressing interleukin-18 and interferon-β exhibit potent antitumor effect against intracranial glioma in rats

  • Authors:
    • Gang Xu
    • Yanwu Guo
    • Zhiyuan Seng
    • Gang Cui
    • Jianqiang Qu
  • View Affiliations

  • Published online on: August 5, 2015     https://doi.org/10.3892/or.2015.4174
  • Pages: 1915-1922
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Bone marrow-derived mesenchymal stem cells (BMSCs) are promising gene vehicles for cancer gene therapy. In our previous study, we reported that BMSCs expressing interleukin (IL)-18 effectively inhibit the growth of glioma in rats. In the present study, we further detected the effect of BMSCs co-expressing IL-18 and interferon (IFN)-β, both of which are immunostimulatory cytokines. BMSCs were genetically engineered to express IL-18 and IFN-β by transfection of recombinant lentivirus-mediated gene transfer. Results showed that BMSCs co-expressing the two cytokines displayed more significant inhibition effect on glioma cell growth in vitro when compared with BMSCs solely expressing IL-18 or IFN-β. Treatment of BMSCs co-expressing IL-18 and IFN-β significantly prolonged the survival and inhibited tumor growth in a rat intracranial glioma model. Furthermore, these genetically engineered BMSCs remarkably promoted cell apoptosis, antitumor cytokine production and CD4+ and CD8+ T-cell infiltration in intracranial glioma tissues than BMSCs solely expressing IL-18 or IFN-β. Results of the present study suggested that IL-18 and IFN-β had a synergistic effect on glioma inhibition. Moreover, results provided evidence that delivery of IL-18 and IFN-β by BMSCs may be an excellent and promising approach to develop an effective treatment protocol for glioma therapy.
View Figures
View References

Related Articles

Journal Cover

October-2015
Volume 34 Issue 4

Print ISSN: 1021-335X
Online ISSN:1791-2431

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Xu G, Guo Y, Seng Z, Cui G and Qu J: Bone marrow-derived mesenchymal stem cells co‑expressing interleukin-18 and interferon-β exhibit potent antitumor effect against intracranial glioma in rats. Oncol Rep 34: 1915-1922, 2015
APA
Xu, G., Guo, Y., Seng, Z., Cui, G., & Qu, J. (2015). Bone marrow-derived mesenchymal stem cells co‑expressing interleukin-18 and interferon-β exhibit potent antitumor effect against intracranial glioma in rats. Oncology Reports, 34, 1915-1922. https://doi.org/10.3892/or.2015.4174
MLA
Xu, G., Guo, Y., Seng, Z., Cui, G., Qu, J."Bone marrow-derived mesenchymal stem cells co‑expressing interleukin-18 and interferon-β exhibit potent antitumor effect against intracranial glioma in rats". Oncology Reports 34.4 (2015): 1915-1922.
Chicago
Xu, G., Guo, Y., Seng, Z., Cui, G., Qu, J."Bone marrow-derived mesenchymal stem cells co‑expressing interleukin-18 and interferon-β exhibit potent antitumor effect against intracranial glioma in rats". Oncology Reports 34, no. 4 (2015): 1915-1922. https://doi.org/10.3892/or.2015.4174