An in vivo molecular response analysis of colorectal cancer treated with Astragalus membranaceus extract
- Ailun Tseng
- Chih-Hsueh Yang
- Chih-Hao Chen
- Chang-Han Chen
- Shih-Lan Hsu
- Mei-Hsien Lee
- Hoong-Chien Lee
- Li-Jen Su
Affiliations: Institute of Systems Biology and Bioinformatics, National Central University, Taoyuan 320, Taiwan, R.O.C., Graduate Institute of Microbiology and Public Health, National Chung Hsing University, Taichung 402, Taiwan, R.O.C., Institute for Translational Research in Biomedicine, Kaohsiung Chang Gung Memorial Hospital, Kaohsiung 833, Taiwan, R.O.C., Department of Education and Research, Taichung Veterans General Hospital, Taichung 407, Taiwan, R.O.C., Graduate Institute of Pharmacognosy, College of Pharmacy, Taipei Medical University, Taipei 110, Taiwan, R.O.C., Center for Dynamical Biomarkers and Translational Medicine, National Central University, Taoyuan 320, Taiwan, R.O.C.
- Published online on: November 23, 2015 https://doi.org/10.3892/or.2015.4441
Copyright: © Tseng
et al. This is an open access article distributed under the
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The fact that many chemotherapeutic drugs cause chemoresistance and side effects during the course of colorectal cancer treatment necessitates development of novel cytotoxic agents aiming to attenuate new molecular targets. Here, we show that Astragalus membranaceus (Fischer) Bge. var. mongolicus (Bge.) Hsiao (AM), a traditional Chinese medicine, can inhibit tumor growth in vivo and elucidate the underlying molecular mechanisms. The antitumor effect of AM was assessed on the subcutaneous tumors of human colorectal cancer cell line HCT116 grafted into nude mice. The mice were treated with either water or 500 mg/kg AM once per day, before being sacrificed for extraction of tumors, which were then subjected to microarray expression profiling. The gene expression of the extraction was then profiled using microarray analysis. The identified genes differentially expressed between treated mice and controls reveal that administration of AM suppresses chromosome organization, histone modification, and regulation of macromolecule metabolic process. A separate analysis focused on differentially expressed microRNAs revealing involvement of macromolecule metabolism, and intracellular transport, as well as several cancer signaling pathways. For validation, the input of the identified genes to The Library of Integrated Network-based Cellular Signatures led to many chemopreventive agents of natural origin that produce similar gene expression profiles to that of AM. The demonstrated effectiveness of AM suggests a potential therapeutic drug for colorectal cancer.