Open Access

Selenite-induced autophagy antagonizes apoptosis in colorectal cancer cells in vitro and in vivo

  • Authors:
    • Yang Yang
    • Hui Luo
    • Kaiyuan Hui
    • Yali Ci
    • Kejian Shi
    • Ge Chen
    • Lei Shi
    • Caimin Xu
  • View Affiliations

  • Published online on: December 10, 2015     https://doi.org/10.3892/or.2015.4484
  • Pages: 1255-1264
  • Copyright: © Yang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

In the present study, we aimed to investigate the relationship between autophagy and apoptosis in selenite‑treated colorectal cancer (CRC) cells. The effects of selenite on HCT116 and SW480 cell apoptosis were investigated with an Annexin V/propidium iodide (PI) double staining kit by flow cytometry. The punctate of LC3 protein following treatment with selenite was observed by a laser scanning confocal microscope and by transmission electron microscopy. Using western blot assays, we detected the apoptotic and autophagic markers in both CRC cells and mouse xenograft tumor models. We found that sodium selenite induced autophagy in the two CRC cell lines. Consistent with the in vitro results, we observed that the expression of autophagy marker LC3 was increased. Finally, we discovered that modulation of reactive oxygen species by MnTMPyP inhibited autophagy, while H2O2 activated autophagy. These results help to elucidate the anticancer effect of selenium, providing further evidence to exploit novel anticancer drugs targeting selenium.
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March-2016
Volume 35 Issue 3

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Yang Y, Luo H, Hui K, Ci Y, Shi K, Chen G, Shi L and Xu C: Selenite-induced autophagy antagonizes apoptosis in colorectal cancer cells in vitro and in vivo. Oncol Rep 35: 1255-1264, 2016
APA
Yang, Y., Luo, H., Hui, K., Ci, Y., Shi, K., Chen, G. ... Xu, C. (2016). Selenite-induced autophagy antagonizes apoptosis in colorectal cancer cells in vitro and in vivo. Oncology Reports, 35, 1255-1264. https://doi.org/10.3892/or.2015.4484
MLA
Yang, Y., Luo, H., Hui, K., Ci, Y., Shi, K., Chen, G., Shi, L., Xu, C."Selenite-induced autophagy antagonizes apoptosis in colorectal cancer cells in vitro and in vivo". Oncology Reports 35.3 (2016): 1255-1264.
Chicago
Yang, Y., Luo, H., Hui, K., Ci, Y., Shi, K., Chen, G., Shi, L., Xu, C."Selenite-induced autophagy antagonizes apoptosis in colorectal cancer cells in vitro and in vivo". Oncology Reports 35, no. 3 (2016): 1255-1264. https://doi.org/10.3892/or.2015.4484