Leukemia inhibitory factor receptor negatively regulates the metastasis of pancreatic cancer cells in vitro and in vivo

  • Authors:
    • Ding Ma
    • Xiaoqian Jing
    • Baiyong Shen
    • Xinyu Liu
    • Xi Cheng
    • Bingrui Wang
    • Zhiping Fu
    • Chenghong Peng
    • Weihua Qiu
  • View Affiliations

  • Published online on: June 9, 2016     https://doi.org/10.3892/or.2016.4865
  • Pages: 827-836
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Pancreatic cancer (PC) is one of the leading causes of cancer-related deaths worldwide. Frequent metastasis and recurrence are the main reasons for the poor prognosis of PC patients. Thus, the discovery of new biomarkers and wider insights into the mechanisms involved in pancreatic tumorigenesis and metastasis is crucial. In the present study, we report that leukemia inhibitory factor receptor (LIFR) suppresses tumorigenesis and metastasis of PC cells both in vitro and in vivo. LIFR expression was significantly lower in PC tissues and was associated with local invasion (P=0.047), lymph node metastasis (P=0.014) and tumor-node-metastasis (TNM) stage (P=0.002). Overexpression of LIFR significantly suppressed PC cell colony formation (P=0.005), migration (P=0.003), invasion (P=0.010) and wound healing ability (P=0.013) in vitro, while opposing results were observed after LIFR was silenced. Furthermore, animal xenograft and metastasis models confirm that the in vivo results were consistent with the outcomes in vitro. Meanwhile, LIFR inhibited the expression of β-catenin, vimentin and slug and induced the expression of E-cadherin, suggesting that the epithelial-mesenchymal transition regulation pathway may underlie the mechanism. These results indicate that LIFR negatively regulates the metastasis of PC cells.
View Figures
View References

Related Articles

Journal Cover

August-2016
Volume 36 Issue 2

Print ISSN: 1021-335X
Online ISSN:1791-2431

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Ma D, Jing X, Shen B, Liu X, Cheng X, Wang B, Fu Z, Peng C and Qiu W: Leukemia inhibitory factor receptor negatively regulates the metastasis of pancreatic cancer cells in vitro and in vivo. Oncol Rep 36: 827-836, 2016
APA
Ma, D., Jing, X., Shen, B., Liu, X., Cheng, X., Wang, B. ... Qiu, W. (2016). Leukemia inhibitory factor receptor negatively regulates the metastasis of pancreatic cancer cells in vitro and in vivo. Oncology Reports, 36, 827-836. https://doi.org/10.3892/or.2016.4865
MLA
Ma, D., Jing, X., Shen, B., Liu, X., Cheng, X., Wang, B., Fu, Z., Peng, C., Qiu, W."Leukemia inhibitory factor receptor negatively regulates the metastasis of pancreatic cancer cells in vitro and in vivo". Oncology Reports 36.2 (2016): 827-836.
Chicago
Ma, D., Jing, X., Shen, B., Liu, X., Cheng, X., Wang, B., Fu, Z., Peng, C., Qiu, W."Leukemia inhibitory factor receptor negatively regulates the metastasis of pancreatic cancer cells in vitro and in vivo". Oncology Reports 36, no. 2 (2016): 827-836. https://doi.org/10.3892/or.2016.4865