MYO6 knockdown inhibits the growth and induces the apoptosis of prostate cancer cells by decreasing the phosphorylation of ERK1/2 and PRAS40

  • Authors:
    • Dong Wang
    • Libing Zhu
    • Min Liao
    • Tengyue Zeng
    • Wenli Zhuo
    • Shunliang Yang
    • Weizhen Wu
  • View Affiliations

  • Published online on: June 29, 2016     https://doi.org/10.3892/or.2016.4910
  • Pages: 1285-1292
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Prostate cancer is the second most frequently diagnosed cancer among males around the world. Myosin VI (MYO6), as a motor protein, has been reported to be implicated in cancer-related cell migration and cellular functions. To investigate the role of MYO6 in prostate cancer, immunohistochemical analysis was firstly applied to prostate cancer tissues and revealed that MYO6 was closely related with the Gleason score in prostate cancer. Then we used specific short hairpin RNA (shRNA) to downregulate MYO6 expression in DU145 and PC-3 cells and found that decreased MYO6 expression significantly suppressed cell proliferation, as determined by MTT and colony formation assays. Flow cytometry confirmed that the suppression of MYO6 promoted cell cycle arrest at the G2/M and sub-G1 phase in the DU145 cells. Furthermore, PathScan intracellular signaling array analysis demonstrated that the phosphorylation of ERK1/2 and PRAS40 was downregulated in the DU145 cells following MYO6 knockdown. Knockdown of MYO6 downregulated the expression of AKT3 and upregulated the expression of PARP, as confirmed by western blot analysis. These results suggest that MYO6 plays an essential role in the progression of prostate cancer and silencing of MYO6 may be a promising therapeutic approach for prostate cancer.
View Figures
View References

Related Articles

Journal Cover

September-2016
Volume 36 Issue 3

Print ISSN: 1021-335X
Online ISSN:1791-2431

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Wang D, Zhu L, Liao M, Zeng T, Zhuo W, Yang S and Wu W: MYO6 knockdown inhibits the growth and induces the apoptosis of prostate cancer cells by decreasing the phosphorylation of ERK1/2 and PRAS40. Oncol Rep 36: 1285-1292, 2016
APA
Wang, D., Zhu, L., Liao, M., Zeng, T., Zhuo, W., Yang, S., & Wu, W. (2016). MYO6 knockdown inhibits the growth and induces the apoptosis of prostate cancer cells by decreasing the phosphorylation of ERK1/2 and PRAS40. Oncology Reports, 36, 1285-1292. https://doi.org/10.3892/or.2016.4910
MLA
Wang, D., Zhu, L., Liao, M., Zeng, T., Zhuo, W., Yang, S., Wu, W."MYO6 knockdown inhibits the growth and induces the apoptosis of prostate cancer cells by decreasing the phosphorylation of ERK1/2 and PRAS40". Oncology Reports 36.3 (2016): 1285-1292.
Chicago
Wang, D., Zhu, L., Liao, M., Zeng, T., Zhuo, W., Yang, S., Wu, W."MYO6 knockdown inhibits the growth and induces the apoptosis of prostate cancer cells by decreasing the phosphorylation of ERK1/2 and PRAS40". Oncology Reports 36, no. 3 (2016): 1285-1292. https://doi.org/10.3892/or.2016.4910