Circulating tumour cells as biomarkers for evaluating cryosurgery on unresectable hepatocellular carcinoma

  • Authors:
    • Jian Shi
    • Yuan Li
    • Shuzhen Liang
    • Jianying Zeng
    • Guifeng Liu
    • Feng Mu
    • Haibo Li
    • Jibing Chen
    • Mao Lin
    • Shihou Sheng
    • Huaiyu Zhang
    • Tongjun Liu
    • Lizhi Niu
  • View Affiliations

  • Published online on: August 25, 2016     https://doi.org/10.3892/or.2016.5050
  • Pages: 1845-1851
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Abstract

We evaluated the efficacy of pre-cryosurgery and post-cryosurgery circulating tumour cells (CTCs) as biomarkers for unresectable hepatocellular carcinoma (HCC). Real‑time qPCR was used to detect potential biomarker genes in CTCs, and magnetic-activated cell sorting (MACS) and fluorescence‑activated cell sorting (FACS) was performed on 47 patients with hepatocellular cancer who underwent cryosurgery. CTCs in the 47 patients were assessed 1 day before cryosurgery, and 7 and 30 days after cryosurgery. The number of CTCs was 17.70±5.725, 14.64±6.761 and 10.28±5.598, respectively, and this decreased significantly over time (P<0.01). ΔCt values for MAGE-3, survivin and carcinoembryonic antigen (CEA) were elevated significantly compared with those obtained before cryosurgery; 2-ΔΔCt values were <1 before cryosurgery, and were 0.63±1.56, 0.21±0.22 and 0.22±0.34 for MAGE-3, survivin and CEA, respectively, at 7 days after treatment. At 30 days after treatment, 2-ΔΔCt values for MAGE-3, survivin and CEA were 0.24±0.82, 0.03±0.07 and 0.02±0.08, indicating that gene expression in CTCs significantly decreased over time (P<0.01). CTCs were useful biomarkers for evaluating the efficacy of cryosurgery on unresectable HCC.
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October-2016
Volume 36 Issue 4

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Shi J, Li Y, Liang S, Zeng J, Liu G, Mu F, Li H, Chen J, Lin M, Sheng S, Sheng S, et al: Circulating tumour cells as biomarkers for evaluating cryosurgery on unresectable hepatocellular carcinoma. Oncol Rep 36: 1845-1851, 2016
APA
Shi, J., Li, Y., Liang, S., Zeng, J., Liu, G., Mu, F. ... Niu, L. (2016). Circulating tumour cells as biomarkers for evaluating cryosurgery on unresectable hepatocellular carcinoma. Oncology Reports, 36, 1845-1851. https://doi.org/10.3892/or.2016.5050
MLA
Shi, J., Li, Y., Liang, S., Zeng, J., Liu, G., Mu, F., Li, H., Chen, J., Lin, M., Sheng, S., Zhang, H., Liu, T., Niu, L."Circulating tumour cells as biomarkers for evaluating cryosurgery on unresectable hepatocellular carcinoma". Oncology Reports 36.4 (2016): 1845-1851.
Chicago
Shi, J., Li, Y., Liang, S., Zeng, J., Liu, G., Mu, F., Li, H., Chen, J., Lin, M., Sheng, S., Zhang, H., Liu, T., Niu, L."Circulating tumour cells as biomarkers for evaluating cryosurgery on unresectable hepatocellular carcinoma". Oncology Reports 36, no. 4 (2016): 1845-1851. https://doi.org/10.3892/or.2016.5050