Oleuropein inhibits the proliferation and invasion of glioma cells via suppression of the AKT signaling pathway

  • Authors:
    • Ming Liu
    • Jian Wang
    • Bin Huang
    • Anjing Chen
    • Xingang Li
  • View Affiliations

  • Published online on: July 28, 2016     https://doi.org/10.3892/or.2016.4978
  • Pages: 2009-2016
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Abstract

Oleuropein, the main phenolic compound of secoiridoids, has been proven to have inhibitory effects on various types of cancers. However, the antitumor effects and related mechanisms in glioma remain unclear. In the present study, U251 and A172 cells were used to assess the effects of oleuropein. Using cell viability assay, we found that oleuropein greatly inhibited the viability of the U251 and A172 cells. Additionally, flow cytometric apoptosis assay indicated that oleuropein induced the apoptosis of the two cell lines. Consistently, the inhibitory effects of oleuropein on migration and invasion were also observed in vitro. In regards to the mechanism, we found that oleuropein significantly decreased phosphorylation of AKT (p-AKT), accompanied by upregulation of Bax and downregulation of Bcl-2. We also found that there was a decrease in the expression levels of matrix metalloproteinase-2 (MMP-2) and MMP-9 after treatment with oleuropein. Furthermore, a specific phosphatidylinositol 3 kinase (PI3K) inhibitor, LY294002, enhanced the pro-apoptotic and anti-invasive effects induced by oleuropein, which suggested that oleuropein suppressed the growth and invasion of glioma cells via inhibition of AKT activity. Taken together, our results indicated that treatment with oleuropein may be an effective therapy for malignant glioma through suppression of tumor proliferation and invasion by inhibition of the AKT signaling pathway.
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October-2016
Volume 36 Issue 4

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Liu M, Wang J, Huang B, Chen A and Li X: Oleuropein inhibits the proliferation and invasion of glioma cells via suppression of the AKT signaling pathway. Oncol Rep 36: 2009-2016, 2016
APA
Liu, M., Wang, J., Huang, B., Chen, A., & Li, X. (2016). Oleuropein inhibits the proliferation and invasion of glioma cells via suppression of the AKT signaling pathway. Oncology Reports, 36, 2009-2016. https://doi.org/10.3892/or.2016.4978
MLA
Liu, M., Wang, J., Huang, B., Chen, A., Li, X."Oleuropein inhibits the proliferation and invasion of glioma cells via suppression of the AKT signaling pathway". Oncology Reports 36.4 (2016): 2009-2016.
Chicago
Liu, M., Wang, J., Huang, B., Chen, A., Li, X."Oleuropein inhibits the proliferation and invasion of glioma cells via suppression of the AKT signaling pathway". Oncology Reports 36, no. 4 (2016): 2009-2016. https://doi.org/10.3892/or.2016.4978