Effects of an extract of Celtis aetnensis (Tornab.) Strobl twigs on human colon cancer cell cultures

  • Authors:
    • Rosaria Acquaviva
    • Valeria Sorrenti
    • Rosa Santangelo
    • Venera Cardile
    • Barbara Tomasello
    • Giuseppe Malfa
    • Luca Vanella
    • Andrea Amodeo
    • Carlo Genovese
    • Silvana Mastrojeni
    • Michela Pugliese
    • Monica Ragusa
    • Claudia Di Giacomo
  • View Affiliations

  • Published online on: August 24, 2016     https://doi.org/10.3892/or.2016.5035
  • Pages: 2298-2304
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Cancers of the digestive tract, in particular colorectal cancer (CRC), are among those most responsive to dietary modification. Research has shown that approximately 75% of all sporadic cases of CRC are directly influenced by diet. Many natural compounds have been investigated for their potential usefulness as cancer chemopreventive agents as they have been thought to suppress carcinogenesis mainly during the initiation phase due to their radical scavenger activity. Since there is an increasing interest in the in vivo protective effects of natural compounds contained in plants against oxidative damage involved in several human diseases such as cancer, the aim of the present research was to test the effects of a Celtis aetnensis (Tornab.) Strobl twig extract on a human colon carcinoma cell line (Caco2). In order to elucidate the mechanisms of action of this extract, LDH release, GSH content, ROS levels, caspase-3 and γ-GCS expression were also evaluated. The results revealed that the Celtis aetnensis extract reduced the cell viability of the Caco2 cells inducing apoptosis at the lowest concentration and necrosis at higher dosages. In addition, this extract caused an increase in the levels of ROS, a decrease in RSH levels and in the expression of HO-1. The expression of γ-GCS was not modified in the Celtis aetnensis-treated Caco-2 cells. These results suggest an interference of this extract on the oxidant/antioxidant cell balance with consequent cell damage. The present study supports the growing body of data suggesting the bioactivities of Celtis aetnensis (Tornab.) Strobl and its potential impact on cancer therapy and on human health.
View Figures
View References

Related Articles

Journal Cover

October-2016
Volume 36 Issue 4

Print ISSN: 1021-335X
Online ISSN:1791-2431

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Acquaviva R, Sorrenti V, Santangelo R, Cardile V, Tomasello B, Malfa G, Vanella L, Amodeo A, Genovese C, Mastrojeni S, Mastrojeni S, et al: Effects of an extract of Celtis aetnensis (Tornab.) Strobl twigs on human colon cancer cell cultures. Oncol Rep 36: 2298-2304, 2016
APA
Acquaviva, R., Sorrenti, V., Santangelo, R., Cardile, V., Tomasello, B., Malfa, G. ... Di Giacomo, C. (2016). Effects of an extract of Celtis aetnensis (Tornab.) Strobl twigs on human colon cancer cell cultures. Oncology Reports, 36, 2298-2304. https://doi.org/10.3892/or.2016.5035
MLA
Acquaviva, R., Sorrenti, V., Santangelo, R., Cardile, V., Tomasello, B., Malfa, G., Vanella, L., Amodeo, A., Genovese, C., Mastrojeni, S., Pugliese, M., Ragusa, M., Di Giacomo, C."Effects of an extract of Celtis aetnensis (Tornab.) Strobl twigs on human colon cancer cell cultures". Oncology Reports 36.4 (2016): 2298-2304.
Chicago
Acquaviva, R., Sorrenti, V., Santangelo, R., Cardile, V., Tomasello, B., Malfa, G., Vanella, L., Amodeo, A., Genovese, C., Mastrojeni, S., Pugliese, M., Ragusa, M., Di Giacomo, C."Effects of an extract of Celtis aetnensis (Tornab.) Strobl twigs on human colon cancer cell cultures". Oncology Reports 36, no. 4 (2016): 2298-2304. https://doi.org/10.3892/or.2016.5035