Upregulation of microRNA-34a enhances the DDP sensitivity of gastric cancer cells by modulating proliferation and apoptosis via targeting MET

  • Authors:
    • Zhandong Zhang
    • Ye Kong
    • Wei Yang
    • Fei Ma
    • Yonglei Zhang
    • Sheqing Ji
    • Er-Min Ma
    • Hongxing Liu
    • Yongshun Chen
    • Yawei Hua
  • View Affiliations

  • Published online on: August 11, 2016     https://doi.org/10.3892/or.2016.5016
  • Pages: 2391-2397
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Abstract

Cisplatin (DDP) based chemotherapy is still the main strategy of human gastric cancer (GC) treatment. However, drug resistance is a major obstacle for DDP chemotherapy. Recent studies indicated that the resistance could be modulated by the regulation of dysregulated microRNAs (miRs). Previous study also found miR-34a was associated with cell proliferation and apoptosis in human GC; however, the relationship between miR-34a and DDP resistance still remains unexplored. The purpose of this study was to investigate whether miR-34a is associated with DDP resistance in human GC cells. Our study found that the expression of miR-34a was significantly decreased in DDP resistance human GC tissues and DDP resistance human GC SGC7901/DDP cells compared with normal GC tissues and cells. Upregulation of miR-34a enhanced the DDP sensitivity of SGC7901/DDP cells to DDP through the inhibition of cell proliferation and induction of cell apoptosis; on the other hand downregulation of miR-34a could weaken the DDP sensitivity of SGC7901 cells to DDP. Further study found that MET was a direct target of miR-34a and the regulation of MET could affect the DDP sensitivity of SGC7901/DDP cells. Moreover, our study also indicated that up­regulation of miR-34a could decrease the expression of MET in SGC7901/DDP cells. Therefore, our findings suggested miR-34a could modulate human gastric cancer cell DDP sensitivity by regulation of cell proliferation and apoptosis via targeting MET, potentially benefiting human GC treatment in the future.
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October-2016
Volume 36 Issue 4

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Zhang Z, Kong Y, Yang W, Ma F, Zhang Y, Ji S, Ma E, Liu H, Chen Y, Hua Y, Hua Y, et al: Upregulation of microRNA-34a enhances the DDP sensitivity of gastric cancer cells by modulating proliferation and apoptosis via targeting MET. Oncol Rep 36: 2391-2397, 2016
APA
Zhang, Z., Kong, Y., Yang, W., Ma, F., Zhang, Y., Ji, S. ... Hua, Y. (2016). Upregulation of microRNA-34a enhances the DDP sensitivity of gastric cancer cells by modulating proliferation and apoptosis via targeting MET. Oncology Reports, 36, 2391-2397. https://doi.org/10.3892/or.2016.5016
MLA
Zhang, Z., Kong, Y., Yang, W., Ma, F., Zhang, Y., Ji, S., Ma, E., Liu, H., Chen, Y., Hua, Y."Upregulation of microRNA-34a enhances the DDP sensitivity of gastric cancer cells by modulating proliferation and apoptosis via targeting MET". Oncology Reports 36.4 (2016): 2391-2397.
Chicago
Zhang, Z., Kong, Y., Yang, W., Ma, F., Zhang, Y., Ji, S., Ma, E., Liu, H., Chen, Y., Hua, Y."Upregulation of microRNA-34a enhances the DDP sensitivity of gastric cancer cells by modulating proliferation and apoptosis via targeting MET". Oncology Reports 36, no. 4 (2016): 2391-2397. https://doi.org/10.3892/or.2016.5016