Hypermethylation and downregulation of glutathione peroxidase 3 are related to pathogenesis of melanoma

  • Authors:
    • Haiyue Chen
    • Zhenlong Zheng
    • Ki-Yeol Kim
    • Xuemei Jin
    • Mi Ryung Roh
    • Zhehu Jin
  • View Affiliations

  • Published online on: September 5, 2016     https://doi.org/10.3892/or.2016.5071
  • Pages: 2737-2744
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Abstract

As a crucial antioxidant enzyme, glutathione peroxidase 3 (GPX3) has been found to be frequently repressed in many cancers due to promoter hypermethylation and is known as a possible tumor suppressor gene. In the present study, we investigated whether promoter hypermethylation of GPX3 and its repression are present in melanoma and, if so, whether GPX3 downregulation is implicated in the pathogenesis of melanoma. Our results revealed methylation of GPX3 and downregulation of its expression in both melanoma cell lines and surgical melanoma tissue samples. In melanoma cell lines, GPX3 expression was restored by treatment with 5-aza-2'-deoxycytidine both in mRNA and protein levels. Depletion of GPX3 was found to increase the proliferative ability, motility, and invasiveness of melanoma cells. Moreover, negative expression of GPX3 was related to poor prognosis in melanoma patients. These results suggest that methylation-mediated GPX3 repression may have critical implications for melanoma pathogenesis.
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November-2016
Volume 36 Issue 5

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Chen H, Zheng Z, Kim K, Jin X, Roh MR and Jin Z: Hypermethylation and downregulation of glutathione peroxidase 3 are related to pathogenesis of melanoma. Oncol Rep 36: 2737-2744, 2016
APA
Chen, H., Zheng, Z., Kim, K., Jin, X., Roh, M.R., & Jin, Z. (2016). Hypermethylation and downregulation of glutathione peroxidase 3 are related to pathogenesis of melanoma. Oncology Reports, 36, 2737-2744. https://doi.org/10.3892/or.2016.5071
MLA
Chen, H., Zheng, Z., Kim, K., Jin, X., Roh, M. R., Jin, Z."Hypermethylation and downregulation of glutathione peroxidase 3 are related to pathogenesis of melanoma". Oncology Reports 36.5 (2016): 2737-2744.
Chicago
Chen, H., Zheng, Z., Kim, K., Jin, X., Roh, M. R., Jin, Z."Hypermethylation and downregulation of glutathione peroxidase 3 are related to pathogenesis of melanoma". Oncology Reports 36, no. 5 (2016): 2737-2744. https://doi.org/10.3892/or.2016.5071