UII/GPR14 is involved in NF-κB-mediated colonic inflammation in vivo and in vitro

  • Authors:
    • Yi Yang
    • Jinpei Zhang
    • Xi Chen
    • Tao Wu
    • Xin Xu
    • Gang Cao
    • Hua Li
    • Yiming Li
  • View Affiliations

  • Published online on: September 5, 2016     https://doi.org/10.3892/or.2016.5069
  • Pages: 2800-2806
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Abstract

The present study was conducted to investigate the molecular mechanism of urotensin II (UII) and its receptor, G protein‑coupled receptor 14 (GPR14), in colonic inflammation. Urantide, a special antagonist of GPR14, and GPR14-siRNA were used to inhibit GPR14 signaling in dextran sulfate sodium (DSS)‑induced inflammation in mice and Caco-2 cells. The results showed that urantide alleviated rectal bleeding, histological injury and production of interleukin (IL)-17 and tumor necrosis factor‑α (TNF‑α) caused by DSS in mice. GPR14-siRNA transfection subsequent with GPR14 inhibition reduced DSS-induced interferon-γ (IFN)-γ production in Caco-2 cells. Meanwhile, both in vivo and in vitro data demonstrated that inhibition of UII/GPR14 alleviated nuclear factor-κB (NF-κB) activation caused by DSS. In conclusion, UII/GPR14 signaling was involved in the DSS-induced colonic inflammation and its inhibition may serve as a potential therapeutic target, which may be associated with the NF-κB signaling pathway.
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November-2016
Volume 36 Issue 5

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Yang Y, Zhang J, Chen X, Wu T, Xu X, Cao G, Li H and Li Y: UII/GPR14 is involved in NF-κB-mediated colonic inflammation in vivo and in vitro. Oncol Rep 36: 2800-2806, 2016
APA
Yang, Y., Zhang, J., Chen, X., Wu, T., Xu, X., Cao, G. ... Li, Y. (2016). UII/GPR14 is involved in NF-κB-mediated colonic inflammation in vivo and in vitro. Oncology Reports, 36, 2800-2806. https://doi.org/10.3892/or.2016.5069
MLA
Yang, Y., Zhang, J., Chen, X., Wu, T., Xu, X., Cao, G., Li, H., Li, Y."UII/GPR14 is involved in NF-κB-mediated colonic inflammation in vivo and in vitro". Oncology Reports 36.5 (2016): 2800-2806.
Chicago
Yang, Y., Zhang, J., Chen, X., Wu, T., Xu, X., Cao, G., Li, H., Li, Y."UII/GPR14 is involved in NF-κB-mediated colonic inflammation in vivo and in vitro". Oncology Reports 36, no. 5 (2016): 2800-2806. https://doi.org/10.3892/or.2016.5069